PXD063259

PXD063259 is an original dataset announced via ProteomeXchange.

Title	Integrated amino acid profiling and 4D-DIA proteomics reveal protein quality divergence and metabolic adaptation in Cordyceps species
Description	To explore the differences in protein quality among classic medicinal entomopatho-genic fungi and to evaluate their metabolic adaptability, we analyzed the amino acid composition and proteomic characteristics of Cordyceps sinensis (CS), Cordyceps militaris (CM) and Cordyceps cicadae (CC). Quantitative analysis showed CM contained the highest crude protein and lysine, methionine, threonine, and valine. CS adapted to high-altitude hypoxia and exhibited lower protein but elevated leucine, isoleucine, and histidine contents, which may contribute to membrane stabilization and oxidative stress resistance. CC displayed higher non-essential amino acids such as arginine, pro-line, and tyrosine, reflecting active nitrogen metabolism. Four-dimensional da-ta-independent acquisition (4D-DIA) proteomics identified 495 differentially expressed proteins (DEPs), compared with CS, with CM and CC showing upregulated aspartate aminotransferase 2 (AAT2), carbamoyl-phosphate synthase 1 (CPA1), and arginino-succinate synthase 1 (ASS1) in alanine, aspartate, and glutamate pathways, enhancing amino acid biosynthesis. Branched-chain keto acid dehydrogenase E1 subunit alpha (BCKDHA), and acyl-CoA dehydrogenase short/branched chain (ACADSB) in CM and CC drove the integration of branched-chain amino acid catabolism and TCA cycle, which may explain the observed protein content differences between species. This study pioneers the application of 4D-DIA proteomics in comparing CS, CM, and CC, laying a foundation for elucidating quality divergence mechanisms in medicinal fungi.
HostingRepository	iProX
AnnounceDate	2025-04-24
AnnouncementXML	Submission_2025-04-23_19:32:18.955.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuyu Tang
SpeciesList	scientific name: Cordyceps militaris; NCBI TaxID: 73501; scientific name: Ophiocordyceps sinensis; NCBI TaxID: 72228; scientific name: Cordyceps cicadae; NCBI TaxID: 218633;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-23 19:31:52	ID requested
⏵ 1	2025-04-23 19:32:19	announced

Dataset with its publication pending

submitter keyword: Cordyceps sinensis, Cordyceps militaris, Cordyceps cicadae, 4D-DIA proteomic, amino acid

Xiuzhang Li
contact affiliation	Qinghai University
contact email	xiuzhang11@163.com
lab head
Chuyu Tang
contact affiliation	Qinghai University
contact email	chuyutang0410@163.com
dataset submitter

iProX dataset URI