PXD063228

PXD063228 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS of the brain in mice with Alzheimer's disease
Description	The spatiotemporal progression of amyloid protein pathology in Alzheimer's disease (AD) is region-specific, but traditional analytical methods have difficulty in resolving the differences in plaque composition in different brain regions. In this study, a photosensitive probe P5 based on engineered Congo red derivatives was developed. Its specific photocatalytic properties of binding amyloid proteins can achieve precise microdissection of brain slice plaques and subsequent proteomic analysis. Verified by the AD mouse model, this method successfully identified the core biomarkers in hippocampal and cortical sediments. Comparative proteomics revealed significant component overlap in amyloid deposition in the two regions and suggested the key role of the silk autophagy-lysosomal pathway in the pathogenesis of AD. This study provides a new methodological strategy for analyzing region-specific amyloid heterogeneity and its pathological mechanism.
HostingRepository	PRIDE
AnnounceDate	2025-12-16
AnnouncementXML	Submission_2025-12-16_05:46:22.148.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Yan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-23 01:26:09	ID requested
⏵ 1	2025-12-16 05:46:22	announced

Dataset with its publication pending

submitter keyword: amyloid proteins, photosensitizer, photocatalytic proximity labeling, amyloid probe,Alzheimer's disease, congo red

Jing Yan
contact affiliation	The Second Affiliated Hospital of Dalian Medical University, Dalian City, Liaoning Province, China
contact email	yanj05@dmu.edu.cn
lab head
Jing Yan
contact affiliation	大连医科大学
contact email	yanj05@dmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD063228

PRIDE project URI

• PRIDE
  1. PXD063228
     1. Label: PRIDE project
     2. Name: LC-MSMS of the brain in mice with Alzheimer's disease