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PXD063210

PXD063210 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleValidation of antibodies suitable for flow cytometric analysis and immunopeptidomics of peptide-MHC complexes in the outbred Swiss albino mouse strain
DescriptionAntigen presentation on major histocompatibility complex (MHC) molecules is central to the ini-tiation of immune responses and a lot of our understanding about the antigen processing and presentation pathway has been gained through studies in mice. MHC molecules are the most genetically diverse genes, consequently mouse strains differ substantially in their MHC make up and resulting antigen presentation. Swiss mice are commonly used in pharmacological research, yet our understanding of antigen presentation in this strain is surprisingly limited. Here, we have tested a range of anti-MHC antibodies and present a range of clones suitable to analyse MHC class I and II molecules in Swiss mice who have the H2-q MHC haplotype. Moreover, we demonstrate using immunopeptidomics that clones 28-12-8, 34-1-2, MKD6 and N22 are also suited to isolate MHC class I and class II ligands in this mouse strain. Thus, this work also establishes a first experimental account of the H2-q derived thymus and spleen immunopeptidome in Swiss mice which bears strong resemblance with ligands isolated from the H2-d MHC haplotype of Balb/C mice.
HostingRepositoryMassIVE
AnnounceDate2025-04-22
AnnouncementXMLSubmission_2025-04-22_21:59:15.318.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterA. Braun
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListAcetyl; Oxidation; Deamidated
InstrumenttimsTOF Pro 2
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-04-22 20:43:37ID requested
12025-04-22 21:59:15announced
Publication List
no publication
Keyword List
submitter keyword: Swiss mice, Immunopeptidome, immunopeptidomics, MHC, antigen presentation, thymus, spleen, DatasetType:Proteomics, DatasetType:Other (Immunopeptidomics)
Contact List
Asolina Braun
contact affiliationMonash University
contact emailasolina.braun@monash.edu
lab head
A. Braun
contact affiliationMonash
contact emailassolbraun@web.de
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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