PXD063210
PXD063210 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Validation of antibodies suitable for flow cytometric analysis and immunopeptidomics of peptide-MHC complexes in the outbred Swiss albino mouse strain |
Description | Antigen presentation on major histocompatibility complex (MHC) molecules is central to the ini-tiation of immune responses and a lot of our understanding about the antigen processing and presentation pathway has been gained through studies in mice. MHC molecules are the most genetically diverse genes, consequently mouse strains differ substantially in their MHC make up and resulting antigen presentation. Swiss mice are commonly used in pharmacological research, yet our understanding of antigen presentation in this strain is surprisingly limited. Here, we have tested a range of anti-MHC antibodies and present a range of clones suitable to analyse MHC class I and II molecules in Swiss mice who have the H2-q MHC haplotype. Moreover, we demonstrate using immunopeptidomics that clones 28-12-8, 34-1-2, MKD6 and N22 are also suited to isolate MHC class I and class II ligands in this mouse strain. Thus, this work also establishes a first experimental account of the H2-q derived thymus and spleen immunopeptidome in Swiss mice which bears strong resemblance with ligands isolated from the H2-d MHC haplotype of Balb/C mice. |
HostingRepository | MassIVE |
AnnounceDate | 2025-04-22 |
AnnouncementXML | Submission_2025-04-22_21:59:15.318.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | A. Braun |
SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
ModificationList | Acetyl; Oxidation; Deamidated |
Instrument | timsTOF Pro 2 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-04-22 20:43:37 | ID requested | |
⏵ 1 | 2025-04-22 21:59:15 | announced |
Publication List
no publication |
Keyword List
submitter keyword: Swiss mice, Immunopeptidome, immunopeptidomics, MHC, antigen presentation, thymus, spleen, DatasetType:Proteomics, DatasetType:Other (Immunopeptidomics) |
Contact List
Asolina Braun | |
---|---|
contact affiliation | Monash University |
contact email | asolina.braun@monash.edu |
lab head | |
A. Braun | |
contact affiliation | Monash |
contact email | assolbraun@web.de |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097693/ |