PXD063210

PXD063210 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Validation of antibodies suitable for flow cytometric analysis and immunopeptidomics of peptide-MHC complexes in the outbred Swiss albino mouse strain
Description	Antigen presentation on major histocompatibility complex (MHC) molecules is central to the ini-tiation of immune responses and a lot of our understanding about the antigen processing and presentation pathway has been gained through studies in mice. MHC molecules are the most genetically diverse genes, consequently mouse strains differ substantially in their MHC make up and resulting antigen presentation. Swiss mice are commonly used in pharmacological research, yet our understanding of antigen presentation in this strain is surprisingly limited. Here, we have tested a range of anti-MHC antibodies and present a range of clones suitable to analyse MHC class I and II molecules in Swiss mice who have the H2-q MHC haplotype. Moreover, we demonstrate using immunopeptidomics that clones 28-12-8, 34-1-2, MKD6 and N22 are also suited to isolate MHC class I and class II ligands in this mouse strain. Thus, this work also establishes a first experimental account of the H2-q derived thymus and spleen immunopeptidome in Swiss mice which bears strong resemblance with ligands isolated from the H2-d MHC haplotype of Balb/C mice.
HostingRepository	MassIVE
AnnounceDate	2025-04-22
AnnouncementXML	Submission_2025-04-22_21:59:15.318.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	A. Braun
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Acetyl; Oxidation; Deamidated
Instrument	timsTOF Pro 2

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-22 20:43:37	ID requested
⏵ 1	2025-04-22 21:59:15	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Swiss mice, Immunopeptidome, immunopeptidomics, MHC, antigen presentation, thymus, spleen, DatasetType:Proteomics, DatasetType:Other (Immunopeptidomics)

submitter keyword: Swiss mice, Immunopeptidome, immunopeptidomics, MHC, antigen presentation, thymus, spleen, DatasetType:Proteomics, DatasetType:Other (Immunopeptidomics)

Contact List

Asolina Braun
contact affiliation	Monash University
contact email	asolina.braun@monash.edu
lab head
A. Braun
contact affiliation	Monash
contact email	assolbraun@web.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097693/