PXD063083

PXD063083 is an original dataset announced via ProteomeXchange.

Title	Extracellular Vesicles from Candidozyma (Candida) auris inhibit proliferation of CD4 T cells by disrupting the IL-2 Axis
Description	Candidozyma auris (formerly Candida auris) is an emerging multidrug-resistant pathogenic fungus that has rapidly spread across the world. Due to the high frequency of multidrug resistant strains and mortality rate, C. auris is considered a critical health threat by the CDC and the WHO. Like other pathogens, C. auris employs virulence factors that are delivered by extracellular vesicles (EVs). We have shown that EVs from C. auris (CauEVs) activate murine phagocytes, boosting innate immune mechanisms. However, the effect of fungal EVs on lymphoid cells has not yet been addressed. Upon activation, CD4 T cells undergo clonal expansion and cytokine production that orchestrate immune responses to eradicate invading pathogens, a process of critical importance in controlling invasive candidiasis. Here we show that the treatment with CauEVs inhibited the activation-induced CD4 T cells proliferation in a dose-dependent manner. Notably, we found that CauEVs acted at early events downstream to the TCR signaling, inhibiting the MAP kinase phosphorylation. Interestingly, the inhibition of CD4 T cell proliferation by CauEVs was associated with a dramatic increase on the IL-2 production, while blocking the IL-2 downstream signaling. Taken together, our results suggest that CauEVs may contain an immunomodulatory factor(s) that affect the CD4 T cell activation and their fate from early to later events in a previously undescribed mechanism.
HostingRepository	PRIDE
AnnounceDate	2025-12-01
AnnouncementXML	Submission_2025-12-01_08:54:23.849.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simone Sidoli
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-17 14:13:05	ID requested
⏵ 1	2025-12-01 08:54:24	announced

Dataset with its publication pending

submitter keyword: IL-2 production, TCR signaling,Candidozyma auris, extracellular vesicles, multidrug-resistant, T cell proliferation, immunomodulation, MAP kinase inhibition, pathogenic fungus, CD4 T cells

Simone Sidoli
contact affiliation	Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, United States
contact email	simone.sidoli@einsteinmed.edu
lab head
Simone Sidoli
contact affiliation	Albert Einstein College of Medicine
contact email	simone.sidoli@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063083
     1. Label: PRIDE project
     2. Name: Extracellular Vesicles from Candidozyma (Candida) auris inhibit proliferation of CD4 T cells by disrupting the IL-2 Axis