PXD062743

PXD062743 is an original dataset announced via ProteomeXchange.

Title	Proteomics characterization of decellularized extracellular matrix for brain
Description	The brain extracellular matrix (ECM) regulates myelin repair and regeneration after demyelination by interacting with neuronal progenitors and immune cells. Therefore, generating and characterizing ECM scaffolds in regions with distinct neuroregenerative capacities is crucial to identify factors that modulate cellular regenerative behavior. We established an effective decellularization protocol for the human neural stem cell (NSC)-rich subventricular zone (SVZ), frontal cortex (FC), and white matter (WM), and defined region-specific matrisomes using comparative proteomics. As proof of concept, we investigated the survival and differentiation of NSCs and monocytes within the decellularized scaffolds. NSCs cultured in WM and FC acquired an astrocytic phenotype, while both astrocytic and oligodendrocytic phenotypes were promoted by SVZ scaffolds. Additionally, monocytes seeded on SVZ and WM scaffolds exhibited an anti-inflammatory differentiation. This model is suitable for investigating ECM properties and assessing cell-matrix interactions.
HostingRepository	PRIDE
AnnounceDate	2025-05-27
AnnouncementXML	Submission_2025-05-27_07:30:16.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Klein
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-hydroxyproline; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-08 09:28:51	ID requested
⏵ 1	2025-05-27 07:30:17	announced

Dataset with its publication pending

submitter keyword: ECM, Proteomis,Human Brain

Roemel Jeusep
contact affiliation	Experimental and Clinical Research Center, a cooperation between Max Delbrück Center for Molecular Medicine in the Helmholtz Association and Charité Universitätsmedizin Berlin, Berlin, Germany. Humboldt-Universität zu Berlin, Faculty of Life Sciences Berlin, Germany Berlin Institute of Health (BIH), Center for Regenerative Therapies, Berlin, Germany
contact email	oliver.klein@bih-charite.de
lab head
Oliver Klein
contact affiliation	Berlin Institute of Health at Charité
contact email	oliver.klein@charite.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD062743

PRIDE project URI

• PRIDE
  1. PXD062743
     1. Label: PRIDE project
     2. Name: Proteomics characterization of decellularized extracellular matrix for brain