PXD062646

PXD062646 is an original dataset announced via ProteomeXchange.

Title	Metabolic Reprogramming Driven by Ant2 Deficiency Augments T Cell Function and Anti-Tumor Immunity
Description	cell activation demands a significant boost in NAD+ levels, often outpacing the capacity of oxidative phosphorylation (OXPHOS). To explore how T cells manage this metabolic stress, we created T cell-specific ADP/ATP translocase-2 knockout (Ant2-/-) mice. Ant2, which is vital for ADP/ATP exchange between the mitochondria and cytoplasm, when deleted, disrupts OXPHOS by limiting ATP synthase function and blocking NAD+ replenishment. Surprisingly, Ant2-/- naïve T cells show increased activation, proliferation, and effector functions compared to wild-type T cells. Through metabolic profiling, we find these cells adopt a metabolic state similar to that of activated T cells, with heightened mitochondrial biogenesis and anabolic processes. Inhibiting ANT pharmacologically in wild-type T cells mirrors the Ant2-/- phenotype and enhances the effectiveness of adoptive T cell therapies in cancer treatment. These results suggest that Ant2-deficient T cells bypass the usual metabolic shifts necessary for activation, leading to greater T cell function and highlighting ANT inhibition as a potential therapeutic strategy for modulating immune responses.
HostingRepository	PRIDE
AnnounceDate	2025-04-09
AnnouncementXML	Submission_2025-04-09_06:04:21.258.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Berger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-06 02:41:01	ID requested
⏵ 1	2025-04-09 06:04:21	announced

Dataset with its publication pending

submitter keyword: naive T cells,Mouse, Ant2ko, spleen

Michael Berger
contact affiliation	The Institute for Medical Research Israel-Canada, The Hebrew University Medical School, Jerusalem, Israel
contact email	michaelb@ekmd.huji.ac.il
lab head
Michael Berger
contact affiliation	The Lautenberg Center for Immunology and Cancer Research Institute for Medical Research Israel-Canada (IMRIC), Faculty of Medicine The Hebrew University of Jerusalem
contact email	michaelb@ekmd.huji.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD062646

PRIDE project URI

• PRIDE
  1. PXD062646
     1. Label: PRIDE project
     2. Name: Metabolic Reprogramming Driven by Ant2 Deficiency Augments T Cell Function and Anti-Tumor Immunity