PXD062543

PXD062543 is an original dataset announced via ProteomeXchange.

Title	Proximity proteomics of T-tubules
Description	To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an Amph construct fused with miniTurbo (mnTb). We expressed either Amph-mnTb or mnTb alone in muscles, and larvae were reared in the presence or absence of biotin. As a control, LacZ was expressed instead of the mnTb fused constructs. Biotinylated peptides were extracted purified from trypsin-digested samples using Tamavidin 2-REV beads and analyzed by mass spectrometry.
HostingRepository	jPOST
AnnounceDate	2025-11-22
AnnouncementXML	Submission_2025-11-22_00:00:50.762.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harunori Yoshikawa
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; N6-biotinyl-L-lysine
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-02 20:56:46	ID requested
⏵ 1	2025-11-22 00:00:51	announced

Dataset with its publication pending

submitter keyword: T-tubules, Biotinylated Peptides, BioID

Fujita Naonobu
lab head
Harunori Yoshikawa
contact affiliation	Institute for Advanced Biosciences, Keio University
dataset submitter

jPOST dataset URI

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