PXD062324 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Split intein mdx skeletal muscle proteomics |
| Description | Duchenne muscular dystrophy is a rare and lethal neuromuscular disease caused by loss-of-function mutations in the dystrophin protein that provides structural integrity to striated muscle fibers. Mice with loss-of-function mutations for the Dmd gene encoding dystrophin (mdx-4cv) were treated with microdystrophin or split-intein AAV constructs to restore various-length dystrophin isoforms to the skeletal muscle compartment. The aim of this study was to compare protein expression profiles between healthy (WT), mdx-4cv, and AAV-treated mdx-4cv gastrocnemius skeletal muscle. We employed an isobaric labeling TMT multiplex discovery proteomics approach to describe and compare proteomic profiles across experimental groups. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-06 |
| AnnouncementXML | Submission_2025-11-06_11:19:59.774.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Erynn Johnson |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-27 15:25:35 | ID requested | |
| ⏵ 1 | 2025-11-06 11:20:00 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: AAV therapy, split-intein,Duchenne muscular dystrophy, skeletal muscle, LCMS |
Contact List
| James M. Ervasti |
|---|
| contact affiliation | University of Minnesota College of Biological Sciences, Minneapolis, MN 55455 |
| contact email | jervasti@umn.edu |
| lab head | |
| Erynn Johnson |
|---|
| contact affiliation | University of Minnesota |
| contact email | joh18358@umn.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD062324 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD062324
- Label: PRIDE project
- Name: Split intein mdx skeletal muscle proteomics
