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PXD062254

PXD062254 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeep palaeoproteomic profiling of archaeological human brains
DescriptionPalaeoproteomics leverages the persistence, diversity, and biological import of ancient proteins to explore the past, and answer fundamental questions about phylogeny, environment, diet, and disease. These insights are largely gleaned from hard tissues like bone and teeth, as well-established protocols exist for extracting ancient proteins from mineralised tissues. No such method, however, exists for the soft tissues, which are underexplored in palaeoproteomics given permission for destructive analysis routinely depends on a proven methodology. Considering less than one-tenth of all human proteins are expressed in bone, compared to three-quarters in the internal organs, the amount of biological information presently inaccessible is substantial. We address this omission with an optimised LC-FAIMS-MS/MS workflow yielding the largest, most diverse palaeoproteome yet described. Using archaeological human brains, we test ten protocols with varied chemistries and find that urea lysis effectively disrupts preserved membrane regions to expose low-abundant, intracellular analytes. Further, we show that ion mobility spectrometry improves unique protein identification by as much as 40%, and represents a means of “pre-cleaning” dirty archaeological samples. Our methodology will be useful for improving protein recovery from a range of ancient tissues and depositional environments.
HostingRepositoryPRIDE
AnnounceDate2025-04-29
AnnouncementXMLSubmission_2025-04-29_06:13:32.771.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexandra Morton-Hayward
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Ascend
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-03-26 03:23:22ID requested
12025-04-29 06:13:33announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: brain
palaeoproteomics
LC-MS/MS
ion mobility
FAIMS
Contact List
Roman Fischer
contact affiliationAssociate Professor and Head of Discovery Proteomics Facility
contact emailroman.fischer@ndm.ox.ac.uk
lab head
Alexandra Morton-Hayward
contact affiliationUniversity of Oxford
contact emailalexandra.morton-hayward@earth.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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