PXD062168

PXD062168 is an original dataset announced via ProteomeXchange.

Title	Targeting the UFL1-AKT Cascade Suppresses Triple-Negative Breast Cancer Progression
Description	Triple-negative breast cancer (TNBC) is an aggressive and highly lethal disease with limited therapeutic options. While UFL1-mediated UFMylation has been implicated in various diseases, its role in TNBC remains unexplored. In this study, we demonstrate that AKT1 directly interacts with UFL1 and undergoes UFMylation at Lys189, Lys276, and Lys297. This modification enhances AKT phosphorylation and activation, promoting tumor growth and chemoresistance in TNBC. In turn, AKT phosphorylates UFL1 at Thr426, establishing a positive feedback loop that sustains high activity of both pro-oncogenic regulators in TNBC. Disrupting the UFL1-AKT interaction using the specific peptide PDAU-TAT significantly inhibits TNBC progression both in vitro and in vivo. Clinically, elevated pT426 UFL1 expression significantly correlates with increased pAKT levels in TNBC specimens. These findings uncover a crucial positive feedback loop between UFL1 and AKT that drives TNBC progression and suggest that targeting the UFL1-AKT axis could offer a promising therapeutic strategy for TNBC and potentially other aggressive cancers characterized by upregulated UFL1 and AKT activation.
HostingRepository	PRIDE
AnnounceDate	2025-11-12
AnnouncementXML	Submission_2025-11-12_03:44:21.847.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yalei wen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-24 06:06:17	ID requested
⏵ 1	2025-11-12 03:44:22	announced

Dataset with its publication pending

submitter keyword: UFL1,Triple-Negative Breast Cancer,AKT

Tongzheng Liu
contact affiliation	State Key Laboratory of Bioactive Molecules and Druggability Assessment/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China/College of Pharmacy, Jinan University, Guangzhou, 510632, China
contact email	liutongzheng@jnu.edu.cn
lab head
yalei wen
contact affiliation	Guangdong Second Provincial General Hospital, Postdoctoral Station of Traditional Chinese Medicine, School of Medicine, Jinan University, Guangzhou 510632, China
contact email	491574395@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD062168

PRIDE project URI

• PRIDE
  1. PXD062168
     1. Label: PRIDE project
     2. Name: Targeting the UFL1-AKT Cascade Suppresses Triple-Negative Breast Cancer Progression