PXD061929

PXD061929 is an original dataset announced via ProteomeXchange.

Title	Remodeling of RNA-Binding Proteome and RNA-mediated regulation as a new layer of control of sporulation
Description	Sporulation allows certain bacteria to survive extreme conditions for extended periods posing challenges to public health and food safety. Transcriptional level of regulation relying on σ factors has been well studied in the non-pathogenic model Bacillus subtilis, while post-transcriptional control remains poorly understood. RNA-binding proteins (RBPs) and small non-coding RNAs (sRNAs) modulate gene expression by affecting mRNA stability or translation. Recent studies suggest RNA-mediated regulation plays a role in sporulation, but its networks and interactions remain largely uncharacterized, highlighting the need for further investigation. To address this knowledge gap, we adapted OOPS (Orthogonal Organic Phase Separation), a high-throughput method to specifically purify RNA binding proteome (RBPome). Monitoring its dynamics along sporulation of B. subtilis, we observed RBPome is highly remodeled during sporulation process. Our approach led to the identification of novel RBPs and potential RNA-mediated post-transcriptional regulators of sporulation. This work provides critical insights into the interplay between RNAs and RBPs, advancing understanding of post-transcriptional regulation in Gram-positive spore-forming bacteria and offers new resources for exploring the molecular mechanisms that govern sporulation.
HostingRepository	PRIDE
AnnounceDate	2025-08-13
AnnouncementXML	Submission_2025-08-13_09:13:19.657.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maya BELGHAZI
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-17 09:16:03	ID requested
⏵ 1	2025-08-13 09:13:20	announced

Dataset with its publication pending

submitter keyword: RNA binding proteome, Orthogonal Organic Phase Separation, OOOPS, RBPome, bacillus subtilis,sporulation

Clementine DELAN -FORINO
contact affiliation	Laboratoire de Chimie Bacterienne (UMR7283)- Aix Marseille Universite Institut de Microbiologie de la Mediterranee CNRS, 31 chemin Joseph Aiguier 13009 Marseille, France
contact email	cdelan-forino@imm.cnrs.fr
lab head
Maya BELGHAZI
contact affiliation	CNRS
contact email	maya.belghazi@univ-amu.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD061929

PRIDE project URI

• PRIDE
  1. PXD061929
     1. Label: PRIDE project
     2. Name: Remodeling of RNA-Binding Proteome and RNA-mediated regulation as a new layer of control of sporulation