PXD061830

PXD061830 is an original dataset announced via ProteomeXchange.

Title	SNX10 regulates the proliferation, apoptosis and cell cycle of acute B lymphoblastic leukemia cells via the PI3K/Akt signaling pathway
Description	B-cell acute lymphoblastic leukemia (B-ALL) is a type of acute lymphoblastic leukemia that originates from B cells. It typically occurs in children and adolescents, but it can also appear in adults. Sorting nexin 10 (SNX10) has recently been identified as a significant regulatory factor in various tumors, although its specific roles remain contested. However, its function in B-ALL has not been previously explored. The present study investigated the role of SNX10 in B-ALL pathogenesis. Bioinformatics analysis identified SNX10 as a Core Hub gene in the B-ALL signaling network, with significantly reduced expression in patients with B-ALL. These findings were corroborated through analysis of clinical bone marrow samples and B-ALL cell lines. Functional in vitro studies revealed that SNX10 knockdown markedly inhibited B-ALL cell proliferation, increased apoptosis, and arrested cells in the G0/G1 phase. In contrast, SNX10 overexpression enhanced cell proliferation, suppressed apoptosis and promoted G2/M phase progression. Proteomic analysis further implicated the PI3K/Akt signaling pathway in mediating the effects of SNX10. Specifically, SNX10 overexpression increased the phosphorylation levels of PI3K and Akt, while SNX10 knockdown had the opposite effect. In vivo experiments demonstrated that elevated SNX10 expression accelerated leukemia progression in a mouse model. Collectively, these findings highlighted the pivotal role of SNX10 in promoting B-ALL cell proliferation via the PI3K pathway, highlighting its potential as a therapeutic target for B-ALL and providing a foundation for future investigations.
HostingRepository	iProX
AnnounceDate	2025-03-14
AnnouncementXML	Submission_2025-03-14_04:29:27.200.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chenyu Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-14 04:28:59	ID requested
⏵ 1	2025-03-14 04:29:27	announced

Dataset with its publication pending

submitter keyword: B-cell acute lymphoblastic leukemia, sorting nexin 10, PI3K/Akt signaling pathway, proliferation, apoptosis, cell cycle, bioinformatics analysis

Fang Yu
contact affiliation	Center for Clinical Laboratories, Affiliated Hospital of Guizhou Medical University
contact email	yu.fang@gmc.edu.cn
lab head
Chenyu Wang
contact affiliation	Center for Clinical Laboratories, Affiliated Hospital of Guizhou Medical University
contact email	451415021@qq.com
dataset submitter

iProX dataset URI