PXD061713

PXD061713 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Glycosylation of serine/threonine-rich intrinsically disordered regions of membrane-associated proteins in streptococci
Description	Despite lacking a tertiary structure, intrinsically disordered regions (IDRs) of proteins play a range of functional roles including cell signaling and protein folding in eukaryotes. However, the functions of bacterial IDRs are poorly understood. Here, we identify that streptococci possess a subset of proteins harboring long-extracytoplasmic IDRs enriched with serine/threonine residues that are O-glycosylated with N-acetylgalactosamine (GalNAc) by pgf operon in S. mutans, and glucose by GtrB-glycosyltransferase in S. pyogenes and S. pneumoniae. Peptidyl-prolyl isomerase PrsA and penicillin-binding protein Pbp1A are identified as the major glycoproteins. Furthermore, loss of IDR glycosylation in PrsA resulted in a defect in biofilm formation in S. mutans. Biochemical and functional characterization demonstrates that IDR does not affect PrsA stability and is protected with GalNAc from proteolysis by an unknown protease in S. mutans. Also, PrsA expression and the degree of glycosylation in S. mutans strongly depend on the length of IDR.
HostingRepository	MassIVE
AnnounceDate	2025-03-11
AnnouncementXML	Submission_2025-03-11_11:10:40.445.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mohammad Rahman
SpeciesList	scientific name: Streptococcus mutans; NCBI TaxID: 1309; scientific name: Streptococcus pneumoniae TIGR4; NCBI TaxID: 170187; scientific name: Streptococcus pneumoniae D39; NCBI TaxID: 373153; scientific name: Streptococcus pyogenes MGAS2111; NCBI TaxID: 561522;
ModificationList	unknown modification
Instrument	Orbitrap Exploris 480; LTQ Orbitrap Velos Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-11 10:44:46	ID requested
⏵ 1	2025-03-11 11:10:40	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Glycosylation, Intrinsically disordered region, DatasetType:Proteomics

submitter keyword: Glycosylation, Intrinsically disordered region, DatasetType:Proteomics

Contact List

Natalia Korotkova
contact affiliation	University of Kentucky
contact email	nkorotkova@uky.edu
lab head
Mohammad Rahman
contact affiliation	University of Kentucky
contact email	mohammad.mizanur.rahman@uky.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v09/MSV000097289/