PXD061514

PXD061514 is an original dataset announced via ProteomeXchange.

Title	Analysis of posttranslational modifications in the Vesicular Glutamate Transporter 2 (Slc17a6)
Description	In contrast to most secondary active transporters, glutamate uptake into synaptic vesicles is not coupled to an ion but driven instead by membrane potential and regulated allosterically by protons and chloride. To understand the mechanisms, we determined high-resolution criyo-EM structures of VGLUT2 and a mutant in complex with a substrate analogue. We identify previously unrecognized palmitoylation that influences recycling of the transporter. We then used mass spectrometry to identity the lipid modification and the sites modified. We found modification of three N-terminal cysteines (C60, C62 and C64) by palmitic acid.
HostingRepository	PRIDE
AnnounceDate	2025-04-16
AnnouncementXML	Submission_2025-04-16_11:20:23.817.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Oses-Prieto
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	palmitoylated-L-cysteine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-05 13:13:10	ID requested
⏵ 1	2025-04-16 11:20:24	announced

Dataset with its publication pending

submitter keyword: Palmitoyl cysteine, rat, VGLUT2

Robert H. Edwards
contact affiliation	Departments of Neurology and Physiology, UCSF School of Medicine. United States
contact email	robert.edwards@ucsf.edu
lab head
Juan Oses-Prieto
contact affiliation	UCSF
contact email	joses@cgl.ucsf.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061514
     1. Label: PRIDE project
     2. Name: Analysis of posttranslational modifications in the Vesicular Glutamate Transporter 2 (Slc17a6)