PXD061393

PXD061393 is an original dataset announced via ProteomeXchange.

Title	Zombosomes are anucleated cell couriers that spread α-synuclein pathology
Description	Astrocytes play a central role in orchestrating the brains microenvironment, but are also tightly connected to neurodegenerative processes. Hence, unraveling astrocytes’ intercellular pathways can give important insight into disease spreading mechanisms. Here we describe a completely new form of actively migrating cellular vehicles that we named zombosomes. Zombosomes shed from astrocytes, but retain their adhesive and motile properties, even though they lack nuclei. Comprehensive analysis of their content revealed that they share protein markers with their parental astrocytes and can be loaded with intact organelles. Importantly, zombosomes act as disease couriers, transferring α-synuclein aggregates from one cell to another, and have the capacity to infiltrate and induce pathology in cerebral organoids. Analysis of human brain sections showed that zombosomes are especially frequent around corpora amyalcea and can carry aggregated α-synuclein. Considering the zombosomes’ content and motility, they propose a likely source of corpora amylacea and the pathological proteins that they harbor. Taken together, our findings represent a new interaction pathway between distant cells through “live” vesicles that when misused, can result in Parkinson’s disease propagation.
HostingRepository	PRIDE
AnnounceDate	2025-12-04
AnnouncementXML	Submission_2025-12-04_01:33:33.929.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Fuchs
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-03 06:01:25	ID requested
⏵ 1	2025-12-04 01:33:34	announced

Dataset with its publication pending

submitter keyword: Disease propagation, Organoids, Corpora Amylacea, Parkinson's disease., Human brain, Astrocyte,Alpha-synuclein

Anna Erlandsson
contact affiliation	Department of Public Health and Caring Sciences, Uppsala University, Sweden
contact email	anna.erlandsson@uu.se
lab head
Johannes Fuchs
contact affiliation	Gothenburg University
contact email	johannes.fuchs@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD061393

PRIDE project URI

• PRIDE
  1. PXD061393
     1. Label: PRIDE project
     2. Name: Zombosomes are anucleated cell couriers that spread α-synuclein pathology