PXD061225

PXD061225 is an original dataset announced via ProteomeXchange.

Title	Rehydration rescues Il22-/- mice from lethal Citrobacter rodentium infection
Description	Interleukin-22 (IL-22) is considered indispensable for host defence against Citrobacter rodentium (CR), with 100% mortality of Il22-/- mice post infection. While IL-22 promotes epithelial barrier integrity and antimicrobial peptide production, the precise mechanism underlying Il22-/- lethality remains unclear. Here, we show that Il22-/- mice succumb to CR infection due to dehydration rather than uncontrolled bacterial burden or inability to regenerate intestinal epithelium. Proteomic analysis at 9 days post infection (dpi) revealed significant downregulation of ion transporters (Slc26a3, Aqp8, Ca2, Ca4, Slc5a8, Pept1) in Il22-/- colonic epithelial cells, suggesting an association between IL-22 deficiency and impaired fluid-electrolyte balance. Fluid therapy (FT), initiated at 5 dpi and lasted for 2 weeks, fully rescued Il22-/- mice, restoring survival without reducing bacterial burden, or affecting immune responses or epithelial integrity. Recovered Il22-/- mice exhibited epithelial regeneration and protection against reinfection, demonstrating that IL-22-independent pathways support long-term mucosal recovery. Notably, advanced AI models consistently failed to predict IL-22’s dispensability. These findings overturn the long-standing paradigm that IL-22 is indispensable for recovery from enteric infection, suggesting that alternative mechanisms can drive epithelial repair and host recovery.
HostingRepository	PRIDE
AnnounceDate	2025-10-25
AnnouncementXML	Submission_2025-10-25_00:01:24.171.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Graeme Benstead-Hume
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-26 06:26:02	ID requested
⏵ 1	2025-10-25 00:01:24	announced

Dataset with its publication pending

submitter keyword: Il22-/-, Citrobacter, rodentium

Jyoti Choudhary
contact affiliation	ICR, Functional Proteomics
contact email	jyoti.choudhary@icr.ac.uk
lab head
Graeme Benstead-Hume
contact affiliation	ICR
contact email	ghume@icr.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061225
     1. Label: PRIDE project
     2. Name: Rehydration rescues Il22-/- mice from lethal Citrobacter rodentium infection