PXD060891

PXD060891 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A Comparison of SWATH-MS Methods for Measurement of Residual Host Cell Proteins in Adeno-Associated Virus Preparations
Description	Analysis of residual host cell proteins (HCPs) in adeno-associated virus (AAV) preparations is challenging due to low availability and high complexity of samples. To address these challenges, sequential window acquisition of all theoretical fragment ion mass spectra (SWATH-MS) data was acquired on four AAV serotypes (2, 5, 8, and 9) produced with HEK293 cells and purified using POROS™ CaptureSelect™ AAVX affinity chromatography with two different analysis software (Skyline and DIA-NN) and instruments (Sciex TripleTOF 6600 and ZenoTOF 7600). An improved liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods well-suited for rAAV HCP analytics was developed by comparing number of protein and precursor identification, HCP quantification and quantification coefficient of variance (CV).
HostingRepository	jPOST
AnnounceDate	2025-04-23
AnnouncementXML	Submission_2025-04-23_06:13:10.128.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lie Min
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-17 14:06:10	ID requested
⏵ 1	2025-04-23 06:13:10	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Host cell protein, Adeno-associated virus, Mass spectrometry, SWATH-MS, DIA-NN, Data independent acquisition, LC-MS/MS

submitter keyword: Host cell protein, Adeno-associated virus, Mass spectrometry, SWATH-MS, DIA-NN, Data independent acquisition, LC-MS/MS

Contact List

Kelvin H. Lee
lab head
Lie Min
contact affiliation	University of Delware
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003617/