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PXD060658

PXD060658 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic and Metabolomic Insights into the Impact of Topping Treatment on Cigar Tobacco
DescriptionTop removal is a widely utilized method in production process of tobacco, but little is known regarding the way it impacts protein and metabolic regulation. In this study, we investigated the underlying processes of alterations in cigar tobacco leaves with and without top removal, using a combined proteomic and metabolomic approach. The results revealed that: (1) Topping significantly affected superoxide anion (O2 -) levels, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content, (2) In the cigar tobacco proteome, 385 differentially expressed proteins (DEPs) were identified, with 228 proteins upregulated and 156 downregulated. Key pathways enriched included flavonoid biosynthesis, porphyrin and chlorophyll metabolism, cysteine and methionine metabolism, and amino acid biosynthesis and metabolism. A network of 161 nodes interconnected by 102 significantly altered proteins was established, (3) In the cigar tobacco metabolome, 247 significantly different metabolites (DEMs) were identified, with 120 upregulated and 128 downregulated metabolites, mainly comprising lipids and lipid-like molecules, phenylpropanoids and polyketides, organic acids and derivatives, and organic heterocyclic compounds, (4) KEGG pathway enrichment revealed upregulation of proteins such as chalcone synthase (CHS), chalcone isomerase (CHI), naringenin 3-dioxygenase (F3H), and flavonoid 3'-monooxygenase (F3'H), along with metabolites like pinocembrin, kaempferol, trifolin, rutin, and quercetin, enhancing the pathways of 'flavonoid' and 'flavone and flavonol' biosynthesis. This study sheds light on the metabolic and proteomic responses of cigar tobacco after topping.
HostingRepositoryiProX
AnnounceDate2025-02-10
AnnouncementXMLSubmission_2025-02-10_18:02:14.989.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDong Guo
SpeciesList scientific name: Nicotiana tabacum; NCBI TaxID: 4097;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-10 18:01:55ID requested
12025-02-10 18:02:15announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Tobacco, Top removal, Flavonoid biosynthesis, Proteomics, Metabolomics
Contact List
Zhaoliang Geng
contact affiliationHaikou Cigar Research Institute
contact emailzhaolianggeng1104@126.com
lab head
Dong Guo
contact affiliationHaikou Cigar Research Institute
contact email447114649@qq.com
dataset submitter
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iProX dataset URI