PXD060658

PXD060658 is an original dataset announced via ProteomeXchange.

Title	Proteomic and Metabolomic Insights into the Impact of Topping Treatment on Cigar Tobacco
Description	Top removal is a widely utilized method in production process of tobacco, but little is known regarding the way it impacts protein and metabolic regulation. In this study, we investigated the underlying processes of alterations in cigar tobacco leaves with and without top removal, using a combined proteomic and metabolomic approach. The results revealed that: (1) Topping significantly affected superoxide anion (O2 -) levels, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content, (2) In the cigar tobacco proteome, 385 differentially expressed proteins (DEPs) were identified, with 228 proteins upregulated and 156 downregulated. Key pathways enriched included flavonoid biosynthesis, porphyrin and chlorophyll metabolism, cysteine and methionine metabolism, and amino acid biosynthesis and metabolism. A network of 161 nodes interconnected by 102 significantly altered proteins was established, (3) In the cigar tobacco metabolome, 247 significantly different metabolites (DEMs) were identified, with 120 upregulated and 128 downregulated metabolites, mainly comprising lipids and lipid-like molecules, phenylpropanoids and polyketides, organic acids and derivatives, and organic heterocyclic compounds, (4) KEGG pathway enrichment revealed upregulation of proteins such as chalcone synthase (CHS), chalcone isomerase (CHI), naringenin 3-dioxygenase (F3H), and flavonoid 3'-monooxygenase (F3'H), along with metabolites like pinocembrin, kaempferol, trifolin, rutin, and quercetin, enhancing the pathways of 'flavonoid' and 'flavone and flavonol' biosynthesis. This study sheds light on the metabolic and proteomic responses of cigar tobacco after topping.
HostingRepository	iProX
AnnounceDate	2025-02-10
AnnouncementXML	Submission_2025-02-10_18:02:14.989.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong Guo
SpeciesList	scientific name: Nicotiana tabacum; NCBI TaxID: 4097;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-10 18:01:55	ID requested
⏵ 1	2025-02-10 18:02:15	announced

Dataset with its publication pending

submitter keyword: Tobacco, Top removal, Flavonoid biosynthesis, Proteomics, Metabolomics

Zhaoliang Geng
contact affiliation	Haikou Cigar Research Institute
contact email	zhaolianggeng1104@126.com
lab head
Dong Guo
contact affiliation	Haikou Cigar Research Institute
contact email	447114649@qq.com
dataset submitter

iProX dataset URI