PXD060567

PXD060567 is an original dataset announced via ProteomeXchange.

Title	Pentraxin3 exacerbates acute pancreatitis injury by inhibiting oxidative phosphorylation pathway
Description	The presence of acute pancreatitis-induced pancreatic necrosis and systemic inflammation is closely related to the prognosis and severity of the disease. Pentraxin 3 is a mediator that aggravates cell injury, and its activation is closely related to oxidative stress and reactive oxygen species generation. This study aims to explore whether PTX3 regulates AP pancreatic necrosis and reveal its potential mechanism of action. By using AP time gradient transcriptomics, proteomics, and liquid chip combined analysis methods, we screened and found a close association between PTX3 and AP. Subsequently, we constructed a classical cerulein-induced in vivo AP model and utilized a CCK-induced in vitro acinar cell injury model to determine the expression of PTX3 and its regulatory role on AP. The results showed that PTX3 presented a high expression level in the cerulein-induced AP model; while after the intervention of recombinant protein PTX3, more severe pancreatic tissue damage and increased serum amylase were observed; in addition, the expression level of PTX3 was significantly higher than that of other proteins, mitochondrial membrane potential decreased and reactive oxygen species levels increased in the in vitro model and were regulated through the oxidative phosphorylation pathway. In conclusion, multi-omics integrated analysis confirmed the close association between Pentraxin 3 and AP and revealed that it exacerbates acute pancreatitis acinar cell injury by mediating oxidative phosphorylation processes.
HostingRepository	iProX
AnnounceDate	2025-02-06
AnnouncementXML	Submission_2025-02-06_19:32:51.621.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenyue Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-06 19:32:41	ID requested
⏵ 1	2025-02-06 19:32:52	announced

Dataset with its publication pending

submitter keyword: acute pancreatitis, Pentraxin 3, acinar cell injury, oxidative phosphorylation, reactive oxygen species

Weiwei Chen
contact affiliation	Yangzhou University
contact email	cww1984@126.com
lab head
Wenyue Wang
contact affiliation	Yangzhou University
contact email	wangwenyue0215@163.com
dataset submitter

iProX dataset URI