PXD060558

PXD060558 is an original dataset announced via ProteomeXchange.

Title	Global Spinal Cord Peptidome Profiling in Response to Osteoarthritis in Rats
Description	Osteoarthritis (OA) is a complex and increasingly prevalent condition affecting an estimated 600 million people worldwide, significantly diminishing quality of life. Understanding OA as both an inflammatory and neurological disease presents challenges for treatment, with no approved disease-modifying therapies available. The neuroactive effects of OA pain on neuropeptide systems, particularly within the spinal cord, remain underexplored, impeding therapeutic advances. Here, we employed mass spectrometry (MS) to characterize the spinal cord peptidome in a validated rat model of OA pain. Peptidome was analyzed at different time points in the Montreal Induction of Rat Arthritis Testing (MI-RAT©; n = 20) model compared to arthrotomic group (Sham; n = 4) and healthy group (Naive; n = 9). Label-free peptidome profiling using liquid chromatography coupled with tandem MS (LC-MS/MS) revealed dynamic changes in endogenous peptides following OA progression, leading to the identification of 624 peptides derived from 29 prohormone precursors. Our findings indicate substantial changes in peptide levels in the spinal cord, particularly involving neuropeptide substance P and peptides derived from proenkephalin, calcitonin gene-related peptide, and somatostatin. These data offer novel insights into the molecular mechanisms underlying OA-associated pain and highlight potential targets for new therapeutic interventions to treat neurochronic pain conditions.
HostingRepository	PRIDE
AnnounceDate	2025-10-28
AnnouncementXML	Submission_2025-10-28_16:13:12.413.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gaoyuan Lu
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	amidated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-06 14:48:56	ID requested
⏵ 1	2025-10-28 16:13:12	announced

Dataset with its publication pending

submitter keyword: pain, MI-RAT, osteoarthritis, mass spectrometry, neuropeptide,peptidome

Lingjun Li
contact affiliation	School of Pharmacy and Department of Chemistry, University of Wisconsin-Madison
contact email	lingjun.li@wisc.edu
lab head
Gaoyuan Lu
contact affiliation	University of Wisconsin-Madison
contact email	gaoyuan.lu@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060558
     1. Label: PRIDE project
     2. Name: Global Spinal Cord Peptidome Profiling in Response to Osteoarthritis in Rats