PXD060298

PXD060298 is an original dataset announced via ProteomeXchange.

Title	Phosphatase specificity influences the phosphorylation timing of CDK substrates during the cell cycle
Description	Cell cycle events are ordered by cyclin-dependent kinases (CDKs), which phosphorylate hundreds of substrates. Multiple phosphatases oppose these CDK substrates, yet their collective role in regulating phosphorylation timing in vivo remains unclear. Here, we show that four phosphatases (PP2A-B55, PP2A-B56, CDC14, and PP1) each target distinct subsets of CDK substrate sites in vivo in fission yeast, influencing when phosphorylation occurs during G2 and mitosis. On average, sites dephosphorylated by CDC14 and PP2A-B56 are phosphorylated earlier during G2, followed by sites dephosphorylated by PP1 and PP2A-B55. This suggests that these phosphatases set different phosphorylation thresholds at the G2/M transition. Consistent with this, depleting PP2A-B55 or CDC14 accelerates mitotic onset, likely by advancing phosphorylation of their respective CDK substrates, suggesting these phosphorylation thresholds are important for regulating mitotic onset. Our findings establish in vivo phosphatase substrate specificity as a key factor regulating the timing of CDK substrate phosphorylation throughout the cell cycle.
HostingRepository	PRIDE
AnnounceDate	2025-10-05
AnnouncementXML	Submission_2025-10-05_09:28:06.493.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Theresa Zeisner
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: NEWT:1264690;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-28 15:41:39	ID requested
⏵ 1	2025-10-05 09:28:06	announced

Dataset with its publication pending

submitter keyword: Fission yeast, CDK,Cell cycle, Phosphatase

Paul Nurse
contact affiliation	The Francis Crick Institute, Cell Cycle Laboratory
contact email	paul.nurse@crick.ac.uk
lab head
Theresa Zeisner
contact affiliation	The Francis Crick Institute, Cell Cycle Lab
contact email	theresa.zeisner@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060298
     1. Label: PRIDE project
     2. Name: Phosphatase specificity influences the phosphorylation timing of CDK substrates during the cell cycle