PXD060162

PXD060162 is an original dataset announced via ProteomeXchange.

Title	Acetyl-phosphate dependent protein acetylation in Neisseria gonorrhoeae
Description	Neisseria gonorrhoeae also called the gonococcus is the aetiological agent of the sexually transmitted disease gonorrhoea. The gonococcus is an obligate human pathogen and possesses the ability to survive intracellularly by the expression of virulence factors. Protein post-translational modifications are found in all organisms, and are involved in the regulation of the metabolism and gene transcription. In this study we investigated the role of non-enzymatic acetylation by acetyl-phosphate in N. gonorrhoeae. This was achieved through the deletion of the genes from the phosphotransacetylase-acetate kinase pathway (PTA-AKA) that control acetyl-phosphate production. As expected, high levels of protein acetylation were observed in the ΔackA strain. Using LC-MS/MS 59% of the N. gonorrhoeae proteome was identified, and we demonstrated that 48.3% of the proteome was acetylated. With many of the of the acetylated proteins being involved in central metabolism especially in pyruvate utilisation. Grow studies showed that the ΔackA strain was unable to utilise pyruvate as a carbon source, whereas it could grown on glucose as well as the wild-type. Furthermore, a deacetylase enzyme was identified and its gene mutated (Δhdac), this allowed the identification of a number of putative targets for HDAC, including phosphotransacetylase. We found that virulence was altered by the change of acetyl-phosphate concentration, with the ΔackA killing the wax moth larvae as a faster rate that the wild-type, whereas the Δpta strain was non-pathogenic in this model. The data obtained suggest that non-enzymatic protein acetylation in N. gonorrhoeae plays an important role in the central metabolism, carbon source utilisation and virulence of this bacterium.
HostingRepository	PRIDE
AnnounceDate	2026-01-30
AnnouncementXML	Submission_2026-01-30_05:45:25.613.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Collins
SpeciesList	scientific name: Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090); NCBI TaxID: NEWT:242231; scientific name: Neisseria gonorrhoeae; NCBI TaxID: NCBITaxon:485;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-24 07:49:30	ID requested
⏵ 1	2026-01-30 05:45:26	announced

Dataset with its publication pending

submitter keyword: acetylome, acetate kinase.,Neisseria gonorrhoeae, phosphotransacetylase, acetyl-phosphate

Mark Collins
contact affiliation	School of Biosciences University of Sheffield Sheffield, S10 2TN United Kingdom
contact email	mark.collins@sheffield.ac.uk
lab head
Mark Collins
contact affiliation	University of Sheffield
contact email	mark.collins@sheffield.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD060162
     1. Label: PRIDE project
     2. Name: Acetyl-phosphate dependent protein acetylation in Neisseria gonorrhoeae