PXD060146

PXD060146 is an original dataset announced via ProteomeXchange.

Title	Targeted Translation Inhibition of Chloroplast and Mitochondrial mRNAs by Designer Pentatricopeptide Repeat Proteins
Description	Pentatricopeptide repeat (PPR) proteins are crucial for organellar gene expression. To establish a tool for gene expression manipulation in Arabidopsis chloroplasts and genetically inaccessible mitochondria, we engineered designer (dPPR) proteins to specifically inhibit the translation of chloroplast and mitochondrial mRNAs by masking their start codons.Unlike prior methods for targeted downregulation of gene expression, which relied on re-targeting natural PPR proteins to RNA sequences closely related to their original targets, our approach employs a fully synthetic P-type PPR scaffold, programmable to bind any RNA sequence of interest. Here, using dPPR-psbK and dPPR-nad7, we targeted the psbKmRNA in chloroplasts and the nad7 mRNA in mitochondria, respectively. Our results demonstrated that dPPR-psbKeffectively binds and inhibits psbK translation with high specificity, resulting in disrupted PSII supercomplexes and reduced photosynthetic efficiency. Similarly, dPPR-nad7 suppressed nad7 translation, leading to decreased NADH oxidase activity in Complex I and growth retardation. By comparing the phenotypes with tobacco psbK knockouts and bir6-2 mutants, we could exclude any physiologically relevant off-target effects. These findings highlight dPPR proteins as precise tools for targeted translation inhibition, enabling functional studies of organellar genes and offering a novel approach for manipulating mitochondrial gene expression with potential applications across diverse plant species.
HostingRepository	PRIDE
AnnounceDate	2025-03-05
AnnouncementXML	Submission_2025-03-05_03:46:37.568.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Serena Schwenkert
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-24 04:41:48	ID requested
⏵ 1	2025-03-05 03:46:37	announced

Dataset with its publication pending

submitter keyword: Arabidopsis,LC-MSMS

Serena Schwenkert
contact affiliation	MSBioLMU Großhaderner Str. 2-4 82152 Planegg-Martinsried Germany
contact email	serena.schwenkert@lmu.de
lab head
Serena Schwenkert
contact affiliation	LMU Munich
contact email	serena.schwenkert@lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD060146

PRIDE project URI

• PRIDE
  1. PXD060146
     1. Label: PRIDE project
     2. Name: Targeted Translation Inhibition of Chloroplast and Mitochondrial mRNAs by Designer Pentatricopeptide Repeat Proteins