PXD059898

PXD059898 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Disulfide bonds are required for cell division, cell envelope biogenesis and antibiotic resistance proteins in mycobacteria
Description	Mycobacterium, including Mycobacterium tuberculosis, the etiological agent of tuberculosis, have a unique cell envelope critical for their survival and antibacterial resistance. The cell envelope's assembly and maintenance influence permeability, making it a key target against multidrug resistant strains. Disulfide bond (SB) formation is crucial for the folding of cell envelope proteins The DSB pathway in mycobacteria includes two enzymes, DsbA and VKOR, required for survival. Using bioinformatics and cystine prfiling proteomics, we identified cell envelope proteins dependent on DSBs. We validated via in vivo alkylation, that key proteins like LamA (MmpS3, PstP, LpqW, and EmbB rely on DSBs for stability. Their stability is disrupted in the Delta VKOR mutant or by VKOR inhibition. Thus targeting DsbA and VKOR systems could compromise both cell division and mycomembrane integrity. These findings emphasize the potential of DSB inhibition as a novel strategy to combat mycobacterial infections.
HostingRepository	MassIVE
AnnounceDate	2025-07-21
AnnouncementXML	Submission_2025-07-21_10:50:02.186.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emma Doud
SpeciesList	scientific name: Mycobacterium smegmatis str. MC2 155; NCBI TaxID: 246196;
ModificationList	unknown modification; TMTpro
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-16 16:00:10	ID requested
⏵ 1	2025-07-21 10:50:02	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: disulfide bonds, oxidative protein folding, DsbA, VKOR, essential proteins, PstP, mycobacteria, actinobacteria, mycomembrane, PP2C, EmbB, Rv2507, MmpS3, cysteine-profiling mass spectrometry, DatasetType:Proteomics

submitter keyword: disulfide bonds, oxidative protein folding, DsbA, VKOR, essential proteins, PstP, mycobacteria, actinobacteria, mycomembrane, PP2C, EmbB, Rv2507, MmpS3, cysteine-profiling mass spectrometry, DatasetType:Proteomics

Contact List

Cristina Landeta
contact affiliation	Indiana University
contact email	clandeta@iu.edu
lab head
Amber L. Mosley
contact affiliation	Indiana University School of Medicine
contact email	almosley@iu.edu
lab head
Emma Doud
contact affiliation	IUSM
contact email	edoud@iu.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v06/MSV000096877/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v06/MSV000096877/