PXD059839

PXD059839 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Brain-wide measurement of synaptic protein turnover reveals localized plasticity during learning
Description	Cells regulate function by synthesizing and degrading proteins. This turnover ranges from minutes to weeks, as it varies across proteins, cellular compartments, cell types, and tissues. Current methods for tracking protein turnover lack the spatial and temporal resolution needed to investigate these processes, especially in the intact brain, which presents unique challenges. We describe a pulse-chase method (DELTA) for measuring protein turnover with high spatial and temporal resolution throughout the body, including the brain. DELTA relies on rapid covalent capture by HaloTag of fluorophores that were optimized for bioavailability in vivo. The nuclear protein MeCP2 showed brain region-and cell type-specific turnover. The synaptic protein PSD95 was destabilized in specific brain regions by behavioral enrichment. A novel variant of expansion microscopy further facilitated turnover measurements at individual synapses. DELTA enables studies of adaptive and maladaptive plasticity in brain-wide neural circuits.
HostingRepository	MassIVE
AnnounceDate	2025-01-15
AnnouncementXML	Submission_2025-01-15_19:23:50.261.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeffrey N. Savas
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	unknown modification
Instrument	Orbitrap Ascend

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-15 14:13:59	ID requested
⏵ 1	2025-01-15 19:23:50	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Brain, Synaptic protein turnover, learning, DatasetType:Proteomics

submitter keyword: Brain, Synaptic protein turnover, learning, DatasetType:Proteomics

Contact List

Jeffrey N. Savas, PhD
contact affiliation	Department of Neurology Northwestern University
contact email	jeffrey.savas@northwestern.edu
lab head
Jeffrey N. Savas
contact affiliation	Northwestern University
contact email	jeffrey.savas@northwestern.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000096857/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000096857/