PXD059825

PXD059825 is an original dataset announced via ProteomeXchange.

Title	Targeting the mitochondrial recycling dysfunction in systemic lupus erythematosus (SLE) patients NK cells with Hydroxychloroquine (HCQ)
Description	Objectives: Natural Killer (NK) cell dysfunction contributes to systemic lupus erythematosus (SLE) pathogenesis, but its underlying mechanisms remain poorly understood. This study investigates immunometabolic alterations in NK cells from SLE patients and explores therapeutic strategies for their restoration. Methods: We characterized mitochondrial structure and function in NK cells from the peripheral blood of SLE patients and healthy controls using flow cytometry, electron microscopy, and proteomics. Key mitophagy-related gene expressions were quantified using qPCR. The therapeutic effects of hydroxychloroquine (HCQ) on mitochondrial recycling and NK cell function were assessed in vitro. Results: SLE NK cells were characterized by an accumulation of enlarged, hyperpolarized mitochondria with cristae disorganization, and reduced mitophagy. Impaired lysosomal acidification and mtDNA extrusion into the cytosol were also observed. Treatment with hydroxychloroquine restored lysosomal pH, mitochondrial recycling, and NK cell effector functions, including cytokine production and cytotoxicity. Conclusions: This study identifies mitochondrial recycling dysfunction as a driver of NK cell abnormalities in SLE and highlights the potential of HCQ to restore NK cell functionality. These findings provide new insights into the immunometabolic mechanisms underlying SLE and suggest avenues for targeted therapeutic interventions.
HostingRepository	PRIDE
AnnounceDate	2026-01-14
AnnouncementXML	Submission_2026-01-13_23:35:42.057.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Denis Comte
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-15 08:31:54	ID requested
⏵ 1	2026-01-13 23:35:42	announced

Dataset with its publication pending

submitter keyword: mitophagy,Systemic Lupus Erythematosus (SLE), Natural Killer (NK) cells, mitochondrial dysfunction, hydroxychloroquine

Denis Comte
contact affiliation	Centre Hospitalier Universitaire Vaudois, Service de Médecine Interne, BH 10-667, Rue du Bugnon 46, CH-1011 Lausanne
contact email	Denis.Comte@chuv.ch
lab head
Denis Comte
contact affiliation	Deputy physician, Privat-Docent, Senior Lecturer Service of Internal Medecine, Lausanne University Hospital (CHUV) CH-1011 Lausanne Switzerland
contact email	denis.comte@chuv.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059825
     1. Label: PRIDE project
     2. Name: Targeting the mitochondrial recycling dysfunction in systemic lupus erythematosus (SLE) patients NK cells with Hydroxychloroquine (HCQ)