PXD059780

PXD059780 is an original dataset announced via ProteomeXchange.

Title	Proteomics data of extracellular vesicles, which are specifically derived from Leydig cells or testicular macrophage cells respectively within the mouse testis, offer detailed and comprehensive insights into the unique biochemical compositions and potential roles of these vesicles in the testicular microenvironment.
Description	Testosterone synthesis in male mammals occurs predominantly in testicular Leydig cells (LCs), a process that is energetically demanding and results in cellular component damage. Despite this, LCs are long-lived and rarely renew, suggesting the presence of specialized mechanisms to maintain LCs homeostasis under these constraints. Emerging evidence highlights intercellular material transfer as a critical process in various physiological contexts, yet its role in LCs homeostasis and function is largely unexplored. Here, we uncover testosterone production has a causal link to bidirectional materials transfers between LCs and testicular macrophages (tMacs). LCs release extracellular vesicles (EVs) containing defective mitochondria, which are eliminated by CD206hi tMacs in a TREM2-dependent process. Conditional deletion of Trem2 in tMacs disrupts this mitochondrial transfer, leading to impaired testosterone synthesis. Conversely, MHCIIhi tMacs enhance testosterone production by transferring EVs containing functional mitochondria to LCs. This EVs-mediated communication depends on ITGB1-VCAM1 interaction, and conditional deletion of Vcam1 in LCs hinders this mitochondrial transfer, compromising testosterone production. Together, our findings reveal an unrecognized bidirectional mitochondrial transfer loop between LCs and tMacs that safeguards LCs homeostasis and testosterone production. This study offers novel insights into the mechanism of testosterone regulation and suggests potential therapeutic targets for male primary hypogonadism.
HostingRepository	PRIDE
AnnounceDate	2026-01-24
AnnouncementXML	Submission_2026-01-24_08:04:23.271.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suyuan Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-14 06:08:05	ID requested
⏵ 1	2026-01-24 08:04:23	announced

Dataset with its publication pending

submitter keyword: Leydig Cells-EVs

Andy P. Xiang
contact affiliation	Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-sen University, Guangzhou, Guangdong, China.
contact email	xiangp@mail.sysu.edu.cn
lab head
Suyuan Zhang
contact affiliation	Zhongshan School of Medicine, Sun Yat-sen University
contact email	1145051589@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD059780

PRIDE project URI

• PRIDE
  1. PXD059780
     1. Label: PRIDE project
     2. Name: Proteomics data of extracellular vesicles, which are specifically derived from Leydig cells or testicular macrophage cells respectively within the mouse testis, offer detailed and comprehensive insights into the unique biochemical compositions and potential roles of these vesicles in the testicular microenvironment.