PXD059545

PXD059545 is an original dataset announced via ProteomeXchange.

Title	One-carbon fixation via the reductive glycine pathway exceeds yield of the Calvin cycle in bacteria
Description	One-carbon (C1) feedstocks like formate could be energetically efficient substrates for sustainable microbial production of food, fuels and chemicals. Here, we replace the native energy-inefficient Calvin-Benson-Bassham (CBB) cycle in Cupriavidus necator with the more energy-efficient reductive glycine pathway for growth on formate and CO2. In chemostats, our engineered strain reaches a 17% higher biomass yield than the wild type, or any natural formatotroph using the Calvin cycle. This demonstrates the potential of synthetic metabolism to realize sustainable, bio-based production.
HostingRepository	PRIDE
AnnounceDate	2025-02-15
AnnouncementXML	Submission_2025-02-15_00:43:57.470.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha); NCBI TaxID: 381666;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-01-08 04:01:40	ID requested
⏵ 1	2025-02-15 00:43:57	announced

Dataset with its publication pending

submitter keyword: Cupriavidus necator,inefficient Calvin-Benson-Bassham cycle

Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany
contact email	timo.glatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Marburg
contact email	timo.glatter@mpi-marburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD059545

PRIDE project URI

• PRIDE
  1. PXD059545
     1. Label: PRIDE project
     2. Name: One-carbon fixation via the reductive glycine pathway exceeds yield of the Calvin cycle in bacteria