PXD059440
PXD059440 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of disulfide bond-linking sites in biosynthesized platelet factor 4 by establishing a partial reduction method without alkylation |
| Description | In this research, a bacterial expression system was established to efficiently generate recombinant human PF4 (rhPF4), and the accuracy of the sequence was validated by liquid chromatography-high-resolution mass spectrometry. A relatively straightforward alkylation-independent partial reduction approach was developed to identify the two disulfide bond sites in PF4, which are critical for its structural integrity and functionality. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-01-05 |
| AnnouncementXML | Submission_2025-01-05_12:17:31.368.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Wenui Hu |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | unknown modification |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-01-05 12:11:46 | ID requested | |
| ⏵ 1 | 2025-01-05 12:17:31 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: platelet factor, E. coli expression system, disulfide bond, liquid chromatography, high resolution mass spectrometry, DatasetType:Proteomics |
Contact List
| Wenrui Hu | |
|---|---|
| contact affiliation | Beijing University of Chemical Technology |
| contact email | 13856359339@163.com |
| lab head | |
| Wenui Hu | |
| contact affiliation | Beijing University of Chemical Technology |
| contact email | 13856359339@163.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000096779/ |
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