PXD059280

PXD059280 is an original dataset announced via ProteomeXchange.

Title	Integrated Structural and Glycoproteomic Profiling Reveals Protein Conformational Remodeling and Biomarkers Across Alzheimer’s Disease Progression
Description	Alzheimer’s disease (AD) is characterized by progressive neurodegeneration and protein misfolding, yet the structural dynamics of proteins and their post-translational modifications during disease progression remain poorly understood. Here, we present an integrated structural and glycoproteomic analysis of paired serum and cerebrospinal fluid (CSF) samples from individuals across three clinical stages: normal cognition, mild cognitive impairment, and AD. Using limited proteolysis mass spectrometry (LiP-MS) combined with high-field asymmetric waveform ion mobility spectrometry and data-independent acquisition, we identified 54 proteins exhibiting structural alterations, two of which (clusterin and ceruloplasmin) showed structural changes in both serum and CSF. Furthermore, our findings reveal potential crosstalk between protein structural changes and N-glycosylation, supported by correlations between LiP-derived structural features and glycosylation patterns in key proteins, such as haptoglobin and kininogen-1. This study demonstrates that integrating structural proteomics with glycoproteomics in matched serum and CSF samples enhances biomarker discovery and provides novel insights into the molecular mechanisms of AD. Our approach offers a powerful platform for identifying robust, minimally invasive biomarkers and for understanding post-translational modification–induced protein remodeling in neurodegenerative diseases.
HostingRepository	PRIDE
AnnounceDate	2026-01-02
AnnouncementXML	Submission_2026-01-02_08:11:10.785.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haiyan Lu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-27 19:17:52	ID requested
⏵ 1	2026-01-02 08:11:11	announced

Dataset with its publication pending

submitter keyword: None

Lingjun Li
contact affiliation	University of Wisconsin-Madison
contact email	lingjun.li@wisc.edu
lab head
Haiyan Lu
contact affiliation	University of Wisconsin-Madison
contact email	luhaiyan2017@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059280
     1. Label: PRIDE project
     2. Name: Integrated Structural and Glycoproteomic Profiling Reveals Protein Conformational Remodeling and Biomarkers Across Alzheimer’s Disease Progression