PXD059267

PXD059267 is an original dataset announced via ProteomeXchange.

Title	Distribution of big tau isoforms in the human central and peripheral nervous system
Description	‘Big tau’, a longer isoform of tau is expressed in peripheral nervous system (PNS) and specific regions of the central nervous system (CNS). Big tau distribution across the human nervous system and pathophysiological role remains largely unknown. Here, using mass spectrometry, we demonstrate the big tau protein results from the insertion of exon 4a-long in the human MAPT sequence. We observed a central-to-peripheral gradient of big tau expression, lowest in the cortical brain region, followed by the cerebellum, then spinal cord, with the highest level in the human sciatic nerve (PNS). Interestingly, brain regions overlapping with relatively lower big tau are more susceptible to neurofibrillary tangle (NFT) formation. Furthermore, we found that CSF big tau levels did not change with CSF Aβ abnormalities in AD, unlike the CNS tau isoform, which increased significantly with concomitant Aβ and cognitive abnormalities. Our findings provide new insights into the basic biology of big tau in humans, crucial for understanding its pathophysiological functions.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:14:18.146.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soumya Mukherjee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-26 11:49:59	ID requested
⏵ 1	2025-09-14 16:14:18	announced

Dataset with its publication pending

submitter keyword: tau protein, nerve, proteomics, brain,Alzheimer's disease, spinal cord, LC-MS/MS

Soumya Mukherjee
contact affiliation	Department of Neurology, Washington University School of Medicine St Louis
contact email	msoumya@wustl.edu
lab head
Soumya Mukherjee
contact affiliation	Assistant Professor
contact email	msoumya@wustl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059267
     1. Label: PRIDE project
     2. Name: Distribution of big tau isoforms in the human central and peripheral nervous system