PXD059092

PXD059092 is an original dataset announced via ProteomeXchange.

Title	Temporal Proteomic Profiling of Pheromone-Induced Cell Cycle Re-Entry in Saccharomyces cerevisiae
Description	The regulation of cell cycle progression in response to environmental cues is essential for cellular adaptation. In Saccharomyces cerevisiae, the BAR1 gene modulates sensitivity to the mating pheromone α-factor, which induces cell cycle arrest. Here we investigated the dynamic proteomic response in the bar1 deletion strain using a 27-plex experimental design with TMTproD isobaric labeling. Asynchronous bar1Δ cells were arrested with α-factor and then released from the pheromone arrest. We acquired three replicate protein abundance time-course profiles following pheromone (α-factor) washout, with samples collected at eight time points from 0 to 165 minutes post-washout. Using higher-order TMTpro sample multiplexing, we generated global temporal profiles of protein abundance associated with recovery from pheromone-induced arrest. Our findings identify specific proteins and pathways involved in cell cycle re-entry and in the attenuation of the pheromone signal, providing insights into the regulatory mechanisms of mating response in yeast. This study contributes significantly to dynamic proteomic analysis in cell cycle progression. We present a powerful approach for investigating complex cellular processes and showcase cell cycle progression following pheromone washout in yeast.
HostingRepository	PRIDE
AnnounceDate	2025-11-24
AnnouncementXML	Submission_2025-11-24_14:08:03.373.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-20 04:30:19	ID requested
⏵ 1	2025-11-24 14:08:03	announced

Dataset with its publication pending

submitter keyword: Eclipse, 35-plex, TMTproD,TMT, α-factor

Joao A. Paulo
contact affiliation	Department of Cell Biology Harvard Medical School Boston, MA, USA
contact email	joao_paulo@hms.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD059092
     1. Label: PRIDE project
     2. Name: Temporal Proteomic Profiling of Pheromone-Induced Cell Cycle Re-Entry in Saccharomyces cerevisiae