PXD058962

PXD058962 is an original dataset announced via ProteomeXchange.

Title	HepG2 cell APOBEC3B-AcGFP anti-AcGFP-IP DIA analysis
Description	APOBEC3B-AcGFP expressing HepG2 or AcGFP expressing HepG2 plated on 10 cm dishes were stimulated with Act D (1 µM), MNNG (50 µM) for 3 h. The cells were washed with PBS three times and lysed with RIPA buffer containing protease inhibitor cocktail. The protein amounts of cell lysates were measure in BCA protein assay. 0.1 mL of the cell lysate (5 mg/mL) were added with 4 µL of Anti GFP (VHH), GFP-Trap Magnetic Agarose and rotated for 1 h at 4˚C. The obtained data were analyzed using DIA-NN 1.8.1. Proteins with both precursor FDR and protein FDR of 1% were identified and quantification values were calculated.
HostingRepository	jPOST
AnnounceDate	2025-12-18
AnnouncementXML	Submission_2025-12-17_07:00:04.621.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yohei Saito
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-17 20:04:58	ID requested
⏵ 1	2025-12-17 07:00:05	announced

Dataset with its publication pending

submitter keyword: APOBEC3B

Yohei Saito
lab head
Yohei Saito
contact affiliation	TMPU
dataset submitter

jPOST dataset URI

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