PXD058703
PXD058703 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In-depth Characterization of S-Glutathionylation of Ventricular Myosin Light Chain 1 Across Species by Top-Down Proteomics |
| Description | S-glutathionylation (SSG) is increasingly recognized as a critical signaling mechanism in the heart, yet SSG modifications in cardiac sarcomeric proteins remain understudied. Here we identified SSG of the ventricular isoform of myosin light chain 1 (MLC-1v) in human, swine, and mouse cardiac tissues using top-down mass spectrometry (MS)-based proteomics. Our results enabled the accurate identification, quantification, and site-specific localization of SSG in MLC-1v across different species. Notably, the endogenous SSG of MLC-1v was observed in human and swine cardiac tissues but not in mice. Treating non-reduced cardiac tissue lysates with GSSG elevated MLC-1v SSG levels across all three species. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-04-01 |
| AnnouncementXML | Submission_2025-04-01_11:55:11.214.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Emily Chapman |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Sus scrofa; common name: pig; NCBI TaxID: 9823; scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | unknown modification |
| Instrument | maXis II; impact II; solariX |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-12-09 11:58:59 | ID requested | |
| ⏵ 1 | 2025-04-01 11:55:11 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: top-down proteomics, s-glutathionylation, sarcomere, myosin light chain, PTM, DatasetType:Proteomics |
Contact List
| Ying Ge | |
|---|---|
| contact affiliation | University of Wisconsin - Madison |
| contact email | ying.ge@wisc.edu |
| lab head | |
| Emily Chapman | |
| contact affiliation | University of Wisconsin - Madison |
| contact email | eachapman2@wisc.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000096622/ |
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