PXD058668

PXD058668 is an original dataset announced via ProteomeXchange.

Title	A subcellular map of translational machinery composition and regulation at the single-molecule level
Description	Millions of ribosomes are packed within mammalian cells, yet we lack tools to visualize them in toto and characterize their subcellular composition. Here, we present ribosome expansion microscopy (RiboExM) to visualize individual ribosomes and an optogenetic proximity-labeling technique (ALIBi) to probe their composition. We generated a super-resolution ribosomal map, revealing unexpected subcellular translational hotspots and enrichment of 60S subunits near polysomes at the endoplasmic reticulum (ER). We found that Lsg1 tethers 60S to the ER and regulates translation of select proteins. Additionally, we discovered ribosome heterogeneity at mitochondria guiding translation of metabolism-related transcripts. Finally, we visualized ribosomes in neurons, revealing a dynamic switch between monosomes and polysomes in neuronal translation. Together, these approaches enable exploration of ribosomal localization and composition at unprecedented resolution.
HostingRepository	PRIDE
AnnounceDate	2025-03-05
AnnouncementXML	Submission_2025-03-05_12:52:53.797.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adele Xu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-08 09:16:40	ID requested
⏵ 1	2025-03-05 12:52:54	announced

Dataset with its publication pending

submitter keyword: ribosome-associated protein,ribosome, ribosome biogenesis, subcellular localization, mRNA translation

Maria Barna
contact affiliation	Department of Genetics, Stanford School of Medicine, USA
contact email	mbarna@stanford.edu
lab head
Adele Xu
contact affiliation	Stanford School of Medicine
contact email	adelexu@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD058668

PRIDE project URI

• PRIDE
  1. PXD058668
     1. Label: PRIDE project
     2. Name: A subcellular map of translational machinery composition and regulation at the single-molecule level