PXD058588

PXD058588 is an original dataset announced via ProteomeXchange.

Title	Wolbachia-mediated reduction in the glutamate receptor mGluR promotes female promiscuity and bacterial spread
Description	The molecular mechanisms by which parasites mediate host behavioral changes remain largely unexplored. Here, we examine Drosophila melanogaster infected with Wolbachia, a symbiont transmitted through the maternal germline, and find Wolbachia infection increases female receptivity to male courtship and hybrid mating. Wolbachia colonize regions of the larval and adult female brain controlling sense perception and behavior. Quantitative global proteomics identified 177 differentially abundant proteins in infected female larval brains. Genetic alteration of the levels of three of these proteins in adults, the metabotropic glutamate receptor mGluR, the transcription factor TfAP-2, and the odorant binding protein Obp99b, each mimicked the effect of Wolbachia on female receptivity. Furthermore, >700 Wolbachia proteins were detected in infected brains. Through abundance and molecular modeling analyses, we distinguished several Wolbachia-produced proteins as potential effectors. These results identify networks of host and Wolbachia proteins that modify behavior to promote mating success and aid the spread of Wolbachia.
HostingRepository	PRIDE
AnnounceDate	2025-04-05
AnnouncementXML	Submission_2025-04-04_16:01:04.887.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tim Karr
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-04 15:28:05	ID requested
⏵ 1	2025-04-04 16:01:05	announced

Dataset with its publication pending

submitter keyword: proteomics,Wolbachia, mating

Tim Karr
contact affiliation	Arizona State University Biodesign Institute Neurodegenerative Disease Research Center
contact email	tkarr@asu.edu
lab head
Tim Karr
contact affiliation	Arizona State University
contact email	tkarr@asu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058588
     1. Label: PRIDE project
     2. Name: Wolbachia-mediated reduction in the glutamate receptor mGluR promotes female promiscuity and bacterial spread