PXD058541

PXD058541 is an original dataset announced via ProteomeXchange.

Title	The phosphoproteomics study of Chk1 and ATR inhibitors regulation on cells
Description	Ataxia Telangiectasia and Rad3-related (ATR) and Checkpoint Kinase 1 (Chk1) are crucial kinases in the DNA Damage Response (DDR) pathway. While the roles of ATR and Chk1 within the DDR are well-established, their roles in mitosis are not fully understood. Here, we describe that the ATR-Chk1 pathway is rewired in mitosis to promote full CDK1 activity, a stark contrast to their role in interphase to inhibit CDK1 following DNA damage. We use mass spectrometry validated the phosphorylation changes after the treatment of ATR and Chk1 inhibitors and confirmed in vitro that Chk1 inhibits residual activity of PKMYT1 (Myt1) via direct phosphorylation at Serine 143.
HostingRepository	PRIDE
AnnounceDate	2025-06-23
AnnouncementXML	Submission_2025-06-23_10:01:24.377.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenxue Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-03 11:10:47	ID requested
⏵ 1	2025-06-23 10:01:24	announced

Dataset with its publication pending

submitter keyword: Phosphoproteomics, CDK1, inhibitor, Chk1

Yansheng Liu
contact affiliation	Yale Cancer Biology Institute
contact email	yansheng.liu@yale.edu
lab head
Wenxue Li
contact affiliation	Yale University
contact email	wenxue.li@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD058541

PRIDE project URI

• PRIDE
  1. PXD058541
     1. Label: PRIDE project
     2. Name: The phosphoproteomics study of Chk1 and ATR inhibitors regulation on cells