PXD058530

PXD058530 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Kidney Tissues Following Ischemia-Reperfusion Injury in Male Mice
Description	Renal ischemia-reperfusion injury (IRI) is a leading cause of acute kidney injury (AKI) and presents significant challenges during kidney transplantation. Due to the detrimental effects of IRI on kidney function and the lack of effective intervention strategies, we conduct a multi-omics study on surgically induced mouse IRI, revealing a modulatory role for the metabolite S-adenosylmethionine (SAM) in AKI development. Our metabolic analysis of clinical samples establishes a link between various AKI conditions and marked elevations in serum SAM, underlying its potential as a biomarker for diagnosis. Furthermore, we find that short-term dietary methionine deprivation, which reduces circulating methionine and kidney SAM levels, effectively enhances renal resilience against IRI. In vivo isotopic tracing demonstrates that this diet preconditions kidney metabolic programs, enhancing glucose and fatty acid oxidation in preparation for IRI. Particularly, the activation of pyruvate dehydrogenase, which produces acetyl-CoA to fuel the tricarboxylic acid (TCA) cycle, highlights an energy-efficient strategy of glucose metabolism that is essential for the protective effects of dietary methionine deprivation.
HostingRepository	PRIDE
AnnounceDate	2025-12-06
AnnouncementXML	Submission_2025-12-05_22:37:41.527.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaofeng Peng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-03 07:26:08	ID requested
⏵ 1	2025-12-05 22:37:42	announced

Dataset with its publication pending

submitter keyword: None

Cunqi Ye
contact affiliation	Zhejiang University
contact email	yecunqi@zju.edu.cn
lab head
Xiaofeng Peng
contact affiliation	Zhejiang University
contact email	xfpeng@zju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD058530

PRIDE project URI

• PRIDE
  1. PXD058530
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Kidney Tissues Following Ischemia-Reperfusion Injury in Male Mice