PXD058381

PXD058381 is an original dataset announced via ProteomeXchange.

Title	Typhoid toxin of Salmonella Typhi elicits host antimicrobial response during acute typhoid fever
Description	Salmonella Typhi secretes typhoid toxin that activates cellular DNA damage responses (DDR) during acute typhoid fever. Human infection challenge studies revealed that the toxin suppresses bacteraemia via unknown mechanisms. By applying proteomics to the plasma of bacteraemic participants, we identified that wild-type toxigenic Salmonella induced release of lysozyme (LYZ) and apolipoprotein C3 (APOC3). LYZ and APOC3 were expressed and secreted in cultured cells treated with purified typhoid toxin or infected with toxigenic Salmonella. Toxin-induced LYZ and APOC3 were dependent upon DDRs and could be inhibited by targeting kinases ATM and ATR. LYZ caused loss of Salmonella morphology characterised by spheroplast formation. Spheroplast formation was mediated by LYZ with EDTA or lactoferrin, which was identified by proteomics in participants with typhoid fever. LYZ alone inhibited secretion of T3SS virulence effector proteins SipB and SopE. Our findings indicate that toxin-induced DDRs elicit antimicrobial responses, which suppress Salmonella bacteraemia during typhoid fever.
HostingRepository	PRIDE
AnnounceDate	2025-10-28
AnnouncementXML	Submission_2025-10-28_07:02:41.725.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Collins
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-28 05:57:44	ID requested
⏵ 1	2025-10-28 07:02:42	announced

Dataset with its publication pending

submitter keyword: typhoid fever,Salmonella Typhi, Human infection challenge

Mark Collins
contact affiliation	School of Biosciences Deputy Director Faculty of Science Mass Spectrometry Centre School of Biosciences University of Sheffield Sheffield, S10 2TN United Kingdom
contact email	mark.collins@sheffield.ac.uk
lab head
Mark Collins
contact affiliation	University of Sheffield
contact email	mark.collins@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD058381

PRIDE project URI

• PRIDE
  1. PXD058381
     1. Label: PRIDE project
     2. Name: Typhoid toxin of Salmonella Typhi elicits host antimicrobial response during acute typhoid fever