PXD058347

PXD058347 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	In-Cell Fast Photochemical Oxidation of Integral Membrane Proteins
Description	We introduce IC-FPOMP (in-cell fast photochemical oxidation of MPs), a method enabling in situ footprinting of IMPs within live cells. IC-FPOMP generates reactive oxygen radicals from several precursors nearby the membrane. We achieve high-throughput and rapid footprinting of IMPs. IC-FPOMP of two human IMPs (human glucose transporter-hGLUT1 and human gamma glutamyl carboxylase-hGGCX) were successful, providing comprehensive footprinting of both the transmembrane and extramembrane regions. IC-FPOMP offers insights on IMP structure-function relationships in cell for the first time, facilitating drug discovery.
HostingRepository	PRIDE
AnnounceDate	2025-02-14
AnnouncementXML	Submission_2025-02-14_13:41:46.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Sun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-27 12:38:16	ID requested
⏵ 1	2025-02-14 13:41:46	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: membrane proteins, footprinting

submitter keyword: membrane proteins, footprinting

Contact List

Michael L.
contact affiliation	Department of Chemistry, Washington University in St. Louis
contact email	mgross@wustl.edu
lab head
Jie Sun
contact affiliation	Washington University in St. Louis
contact email	jie.sun@wustl.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD058347
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD058347

PRIDE project URI

Repository Record List

[ + ]