⮝ Full datasets listing
PXD058151
PXD058151 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mechanosensor-mediated Hsp70 phosphorylation orchestrates the landscape of the heat shock response |
| Description | Yeast must respond rapidly to heat stress by activating multiple signaling pathways that preserve proteostasis. This includes induction of Hsf1 and Msn2/4-mediated transcription, cell integrity signaling, stress-triggered phase separation of proteins and inhibition of translation. How these pathways are so rapidly activated and co-ordinated remains unclear. We show that the mechanosensor Mid2 senses heat-induced membrane stretch and leads to rapid phosphorylation of the cytosolic Hsp70 Ssa1 at a well-conserved threonine (T492). Phosphorylation of T492 leads to epichaperome rearrangement which includes altered interaction with Hsf1, multiple ribosomal subunits, the Bck1 MEK, and Edc3. Taken together these results provide a comprehensive, unified theory of the global yeast shock response that is mediated by the Hsp70 chaperone code. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-17 |
| AnnouncementXML | Submission_2025-11-17_08:36:54.393.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Luca Fornelli |
| SpeciesList | scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932; |
| ModificationList | Acetyl; Phospho |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-11-21 13:53:45 | ID requested | |
| ⏵ 1 | 2025-11-17 08:36:54 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: heat shock response, yeast, Hsp70, DatasetType:Proteomics |
Contact List
| Luca Fornelli | |
|---|---|
| contact affiliation | University of Oklahoma |
| contact email | luca.fornelli@ou.edu |
| lab head | |
| Luca Fornelli | |
| contact affiliation | University of Oklahoma |
| contact email | luca.fornelli@ou.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000096492/ |
