PXD057934

PXD057934 is an original dataset announced via ProteomeXchange.

Title	Distinct Gene Regulatory Dynamics Drive Skeletogenic Cell Fate Convergence During Vertebrate Embryogenesis
Description	Cell type repertoires have expanded extensively in metazoan animals, with some clade-specific cells being paramount to their evolutionary success. A prime example are the skeletogenic cells of the developing vertebrate endoskeleton. Depending on anatomical location, these cells originate from three different embryonic precursor lineages, yet they converge developmentally towards similar cellular phenotypes. Furthermore, these embryonic lineages have gained skeletogenic competency at distinct timepoints during vertebrate evolution, thus questioning to what extent different parts of the vertebrate skeleton rely on truly homologous cell types. Here, we investigate how lineage-specific molecular properties of the three precursor pools are integrated at the gene regulatory level, to allow for phenotypic convergence towards a skeletogenic cell fate. Using single-cell transcriptomics and chromatin accessibility profiling along the precursor-to-skeletogenic cell continuum, we examine the gene regulatory dynamics associated with this cell fate convergence. We find that distinct transcription factor profiles are inherited and integrated from the three precursor states, and that lineage-specific enhancer elements incorporate these different inputs at the cis-regulatory level. This lineage-specific gene regulatory logic for skeletogenic convergence from three embryonic precursor pools suggests that early skeletal cells in different body parts are distinct cell types. Their regulatory uncoupling may render them amenable to individualized selection, to help to define distinct morphologies and biomaterial properties in the different parts of the vertebrate skeleton.
HostingRepository	MassIVE
AnnounceDate	2024-12-05
AnnouncementXML	Submission_2025-01-22_08:36:24.249.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	danilo ritz
SpeciesList	scientific name: Gallus gallus; common name: chicken; NCBI TaxID: 9031;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-15 06:27:34	ID requested
⏵ 1	2025-01-22 08:36:24	announced

no publication

submitter keyword: cell type evolution, cell fate convergence, vertebrate skeletogenesis, gene regulatory evolution, single-cell functional genomics, DatasetType:Proteomics

Alexander Schmidt
contact affiliation	Biozentrum, Universtiy of Basel, 4056 Basel, Switzerland
contact email	alex.schmidt@unibas.ch
lab head
danilo ritz
contact affiliation	University of Basel
contact email	danilo.ritz@unibas.ch
dataset submitter

MassIVE dataset URI

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