PXD057837

PXD057837 is an original dataset announced via ProteomeXchange.

Title	Salt stress activates CDK8-AHL10-SUVH2/9 module to dynamically regulate salt tolerance in Arabidopsis
Description	Salt stress leads to devastating effects to agriculture. Presently, the key regulators that control transcriptional dynamics of salt-responsive genes remain poorly understood in plants. Here, we revealed that salt stress can substantially induce the kinase activity of Mediator subunit cyclin-dependent kinase 8 (CDK8), which is essential for its positive role in regulating salt tolerance. We subsequently uncovered that CDK8 phosphorylates the AT-hook motif nuclear-localized protein 10 (AHL10) at serine 314 through direct interaction, thereby promoting its protein degradation under salt stress. In addition, we created ahl10 mutants by CRISPR-Cas9 and showed the negative role of AHL10 in salt tolerance. Moreover, transcriptome analysis revealed that CDK8 regulates more than 20% of salt-responsive genes, about half of which are co-regulated by AHL10. Chromatin immunoprecipitation sequencing (ChIP-seq) further demonstrated that AHL10 binds to the AT-rich DNA sequence related to the nuclear matrix-attachment regions (MARs) in the salt-responsive gene promoters to repress their transcription. Importantly, we further found that AHL10 physically interacts with SU(VAR)3-9 homologs SUVH2/9, thereby repressing transcription of salt-responsive genes in a H3K9me2-dependent epigenetic regulatory manner. Overall, our study identified the CDK8-AHL10-SUVH2/9 module as a key molecular switch controlling plant transcriptional dynamics in response to salt stress.
HostingRepository	jPOST
AnnounceDate	2025-02-02
AnnouncementXML	Submission_2025-02-22_03:34:52.490.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	tian
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; O18 label
Instrument	instrument

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-13 04:00:43	ID requested
⏵ 1	2025-02-22 03:34:52	announced

Dataset with its publication pending

submitter keyword: salt stress, Mediator subunit, CDK8, AHL10, SUVH, phosphorylation, protein stability, transcriptional regulation

YingFang Zhu
lab head
tian
contact affiliation	Southern University of science and technology
dataset submitter

jPOST dataset URI

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