PXD057743

PXD057743 is an original dataset announced via ProteomeXchange.

Title	FGF13 in septic lung injury: A regulator of the ERK/aerobic glycolysis axis in the inflammatory state
Description	HEK293T cells were transfected with Flag-FGF13 plasmids, and cells were performed with immunoprecipitation by Flag antibody or IgG (CST, 3900, USA) with protein A/G beads (Meck, LSKMAGAG10, USA). Coimmunoprecipitation was carried out in HEK293T cells and visualized with silver staining by using the beyotime fast silver stain kit (P0017S). Then mass spectrometry was used to detect all the proteins connected with Flag-FGF13. The assay was carried by Shanghai Bioprofile Technology Company Ltd. In brief, the gradient consisted of 5~35% (v/v) acetonitrile in 0.1% (v/v) formic acid at a flow rate of 200 nL/min for 10 min, 35~100% (v/v) acetonitrile in 0.1% (v/v) formic acid at a flow rate of 200 nL/min for 2 min and 100% acetonitrile in 0.1% formic acid at a flow rate of 200 nL/min for 8 min. The eluted peptides were ionized and introduced into a Thermo Fisher LTQ Velos Pro mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) using a Proxeon nanoelectrospray ion source. Survey full-scan MS spectra (from m/z 200~1800) were acquired. A full mass spectrum (m/z 200~1800) was followed by fragmentation of the ten most abundant peaks, using 35% of the normalized collision energy for obtaining MS/MS spectra. All peptide assignments were verified by manual inspection. The information about the proteins connected with Flag-FGF13 was summarized in Supplementary materials: Proteins connect with Flag-FGF13.‘’
HostingRepository	PRIDE
AnnounceDate	2025-12-17
AnnouncementXML	Submission_2025-12-17_10:27:03.562.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junjie Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-11 05:51:45	ID requested
⏵ 1	2025-12-17 10:27:04	announced

Dataset with its publication pending

submitter keyword: None

Junjie, Zhu
contact affiliation	School of Pharmaceutical Sciences, Wenzhou Medical University
contact email	zdd12212@126.com
lab head
Junjie Zhu
contact affiliation	School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, 325000, P.R. China.
contact email	zdd12212@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD057743

PRIDE project URI

• PRIDE
  1. PXD057743
     1. Label: PRIDE project
     2. Name: FGF13 in septic lung injury: A regulator of the ERK/aerobic glycolysis axis in the inflammatory state