PXD057583
PXD057583 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mass Spectrometry-Based Proteomics of Extracellular Vesicle Proteins (EVPs) |
| Description | For proteomic analysis, isolated EVPs were subjected to mass spectrometry (MS). Briefly, proteins were extracted from EVP pellets, and concentrations were measured using the Pierce MicroBCA kit. Each sample underwent processing with 20 micrograms of protein, first reduced with DTT, then alkylated with iodoacetamide, and finally digested with trypsin in 25 mM NH4HCO3. The resulting tryptic peptides were cleaned using a C18 column and reconstituted in 25 microliters of 0.1% formic acid; 12 microliters of this solution was analyzed by LC-MS/MS over a 110-minute run. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-11-06 |
| AnnouncementXML | Submission_2024-11-06_11:29:57.603.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Nathan |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-11-06 11:20:23 | ID requested | |
| ⏵ 1 | 2024-11-06 11:29:58 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: trophoblast stem cell secretome, extracellular vesicles and particles, exosomes, senescence, aging, DatasetType:Proteomics |
Contact List
| Kotb Abdelmohsen | |
|---|---|
| contact affiliation | Staff Scientist |
| contact email | abdelmohsenk@mail.nih.gov |
| lab head | |
| Nathan | |
| contact affiliation | Temple University |
| contact email | tun37257@temple.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000096320/ |
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