PXD057192

PXD057192 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profiling Reveals Age-Related Changes in Transporter Proteins in the Human Blood-Brain Barrier
Description	The Blood-Brain Barrier (BBB) is a crucial, selective barrier that regulates the entry of molecules including nutrients, environmental toxins, and therapeutic medications into the brain. This function relies heavily on brain endothelial cell proteins, particularly transporters and tight junction proteins. The BBB continues to develop postnatally, adapting its selective barrier function across different developmental phases, and alters with aging and disease. Here we present a global proteomics analysis focused on the ontogeny and aging of proteins in human brain microvessels (BMVs), predominantly composed of brain endothelial cells. Our proteomic profiling quantified 6,223 proteins and revealed possible age-related alteration in BBB permeability due to basement membrane component changes through the early developmental stage and age-dependent changes in transporter expression. Notable changes in expression levels were observed with development and age in nutrient transporters and transporters that play critical roles in drug disposition. This research 1) provides important information on the mechanisms that drive changes in the metabolic content of the brain with age and 2) enables the creation of physiologically based pharmacokinetic models for CNS drug distribution across different life stages.
HostingRepository	PRIDE
AnnounceDate	2025-11-28
AnnouncementXML	Submission_2025-11-28_05:56:17.975.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Niklas Handin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-25 04:30:19	ID requested
⏵ 1	2025-11-28 05:56:18	announced

Dataset with its publication pending

submitter keyword: Human, LC-MSMS, brain microvessels, Blood-brain barrier, transporters, MaxQuant, Orbitrap, aging, ontogeny

Per Artursson
contact affiliation	Department of Pharmacy, Uppsala University, Uppsala, Sweden
contact email	per.artursson@farmaci.uu.se
lab head
Niklas Handin
contact affiliation	Uppsala University
contact email	handin_n@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD057192
     1. Label: PRIDE project
     2. Name: Proteomic Profiling Reveals Age-Related Changes in Transporter Proteins in the Human Blood-Brain Barrier