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PXD056792

PXD056792 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBarley grain proteome assessment using multi-environment trial data and machine learning
DescriptionProteomics can be used to assess individual protein abundances, which could reflect genotypic and/or environmental effects and potentially predict grain/malt quality. In this study, 79 barley grain samples from a Californian multi-environment trial were assessed using liquid chromatography-mass spectrometry. A total of 3105 proteins were identified across all samples. Location, genotype, and year explained 26.7%, 17.1%, and 14.3% of variance in the relative abundance of individual proteins, respectively. Sixteen proteins with storage, DNA/RNA binding, or enzymatic functions were found to be significantly higher/lower in abundance (compared to the overall mean) in the Yolo 3 and Imperial Valley locations, Butta 12 and LCS Odyssey genotypes, and 2017-18 and 2021-22 years. Individual protein abundances were reasonably predictive (RMSECV=1.25-2.04%) for total, alcohol-soluble, and malt protein content and malt fine extract. This study illustrates the role of the environment on the barley proteome and the utility of proteomics and machine learning to predict grain/malt quality.
HostingRepositoryMassIVE
AnnounceDate2024-10-14
AnnouncementXMLSubmission_2024-10-14_18:36:26.284.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmittergabriela grigorean
SpeciesList scientific name: Hordeum vulgare; common name: barley; NCBI TaxID: 4513;
ModificationListCarbamidomethyl; Oxidation
InstrumenttimsTOF HT
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-10-14 16:09:10ID requested
12024-10-14 18:36:26announced
Publication List
no publication
Keyword List
submitter keyword: barley, proteomics, malt quality, hordein, timsTOF LC-MS, DatasetType:Proteomics
Contact List
Glen Patrick Fox
contact affiliationUniversity of California, Davis
contact emailgpfox@ucdavis.edu
lab head
Christine Diepenbrock
contact affiliationUniversity of California, Davis
contact emailchdiepenbrock@ucdavis.edu
lab head
gabriela grigorean
contact affiliationucd
contact emailggrigorean@ucdavis.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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