PXD056638

PXD056638 is an original dataset announced via ProteomeXchange.

Title	Quantitative Chemoproteomics Reveals Dopamine’s Protective Modification of Tau
Description	Dopamine (DA) is one of the most important neurotransmitters. Oxidation of DA leads to electrophilic quinone intermediates, which can covalently modify nucleophilic residues in proteins, resulting in “dopamination”. Individual dopaminated protein targets have been studied, most of which were functionally damaged by dopamination. Here, we developed a quantitative chemoproteomic strategy to site-specifically measure protein dopamination in mouse brains. More than 6000 dopamination sites were quantified and IC50 values for 64 hyper-sensitive sites were measured. Among them, hyper-sensitive dopamination of two cysteines in microtubule-associated protein Tau were biochemically validated and functionally characterized to prevent Tau’s pathological amyloid fibrillation and promote Tau-mediated assembly of microtubules. Our study not only provided the first global portrait of dopamination with site-specific resolution, but also discovered a protective role of DA in regulating the function of Tau, which may help advance our understanding of the physiological and pathological role of DA in human brain.
HostingRepository	iProX
AnnounceDate	2024-12-20
AnnouncementXML	Submission_2025-08-31_20:47:37.881.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Youjia Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-10-09 00:25:27	ID requested
⏵ 1	2025-08-31 20:47:38	announced

Dataset with its publication pending

submitter keyword: Activity-based protein profiling, Data-independent acquisition, Dopamine, Tau, Post-translational modification

Chu Wang
contact affiliation	Peking University
contact email	chuwang@pku.edu.cn
lab head
Youjia Wang
contact affiliation	Peking University
contact email	2301110438@pku.edu.cn
dataset submitter

iProX dataset URI