PXD056412

PXD056412 is an original dataset announced via ProteomeXchange.

Title	Adipose tissue protein kinase D (PKD): regulation of signalling networks and its effect on metabolism
Description	The protein kinase D (PKD) family of three highly homologous isoforms (PKD1, PKD2, and PKD3) are implicated as nutrient sensing signalling kinases that regulate the response of adipose and other tissues to the nutrient environment. However, the physiological role of adipose tissue PKD and the cellular signalling targets downstream of adipose tissue PKD are not well characterised. Here we employed phosphoproteomics to elucidate signalling events downstream of PKD activation in differentiated 3T3-L1 adipocytes using a triple isoform siRNA knockdown model. This identified PKD-regulated pathways including insulin and cAMP signalling, which control metabolic responses in adipose tissue.
HostingRepository	PRIDE
AnnounceDate	2025-05-27
AnnouncementXML	Submission_2025-05-26_18:20:21.255.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sean Humphrey
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-30 22:26:55	ID requested
⏵ 1	2025-05-26 18:20:21	announced

Dataset with its publication pending

submitter keyword: Human, adipose tissue, phosphoproteomics

Sean Humphrey
contact affiliation	Stem Cell Medicine, Functional Phosphoproteomics Lab, Murdoch Children's Research Institute, Australia
contact email	sean.humphrey@mcri.edu.au
lab head
Sean Humphrey
contact affiliation	Murdoch Children's Research Institute
contact email	sean.j.humphrey@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD056412

PRIDE project URI

• PRIDE
  1. PXD056412
     1. Label: PRIDE project
     2. Name: Adipose tissue protein kinase D (PKD): regulation of signalling networks and its effect on metabolism