PXD056412 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Adipose tissue protein kinase D (PKD): regulation of signalling networks and its effect on metabolism |
Description | The protein kinase D (PKD) family of three highly homologous isoforms (PKD1, PKD2, and PKD3) are implicated as nutrient sensing signalling kinases that regulate the response of adipose and other tissues to the nutrient environment. However, the physiological role of adipose tissue PKD and the cellular signalling targets downstream of adipose tissue PKD are not well characterised. Here we employed phosphoproteomics to elucidate signalling events downstream of PKD activation in differentiated 3T3-L1 adipocytes using a triple isoform siRNA knockdown model. This identified PKD-regulated pathways including insulin and cAMP signalling, which control metabolic responses in adipose tissue. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-27 |
AnnouncementXML | Submission_2025-05-26_18:20:21.255.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sean Humphrey |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-09-30 22:26:55 | ID requested | |
⏵ 1 | 2025-05-26 18:20:21 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, adipose tissue, phosphoproteomics |
Contact List
Sean Humphrey |
contact affiliation | Stem Cell Medicine, Functional Phosphoproteomics Lab, Murdoch Children's Research Institute, Australia |
contact email | sean.humphrey@mcri.edu.au |
lab head | |
Sean Humphrey |
contact affiliation | Murdoch Children's Research Institute |
contact email | sean.j.humphrey@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD056412
- Label: PRIDE project
- Name: Adipose tissue protein kinase D (PKD): regulation of signalling networks and its effect on metabolism