PXD056407
PXD056407 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Establishing a Top-Down Proteomics Platform on a Time-of-Flight Instrument with Electron-Activated Dissociation |
| Description | . Top-down proteomics is the study of intact proteins and their post-translational modifications with mass spectrometry. Historically, this field is more challenging than its bottom-up counterpart because the species are much bigger and have a larger number of possible combinations of sequences and modifications, thus there is a great need for technological development. With improvements in instrumentation and a multiplicity of fragmentation modes available, top-down proteomics is quickly gaining in popularity with renewed attention on increasing confidence in identification and quantification. Here, we systematically evaluated the Sciex ZenoTOF 7600 system for top-down proteomics, applying standards in the field to validate the platform and further experimenting with its capabilities in electron-activated dissociation and post-translational modification site localization. The instrument demonstrated robustness in standard proteins for platform QC, as aided by zeno-trapping. We were also able to apply this to histone post-translational modifications, achieving high sequence coverage that allowed PTMs site-localization across protein sequences with optimized EAD fragmentation. We demonstrated the ability to analyze proteins spanning the mass range and included analysis of glycosylated proteins. This is a reference point for future top-down proteomics experiments to be conducted on the ZenoTOF 7600 system. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-02-17 |
| AnnouncementXML | Submission_2025-02-17_09:35:20.227.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Richard Searfoss |
| SpeciesList | scientific name: Bovinae; NCBI TaxID: 27592; |
| ModificationList | Methyl; Trimethyl; Acetyl; mannosylated |
| Instrument | ZenoTOF 7600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-09-30 15:16:41 | ID requested | |
| ⏵ 1 | 2025-02-17 09:35:20 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Top-Down Proteomics |
Contact List
| Benjamin A. Garcia | |
|---|---|
| contact affiliation | Department of Biochemistry and Biophysics, University of Pennsylvania |
| contact email | bgarci@mail.med.upenn.edu |
| lab head | |
| Richard Searfoss | |
| contact affiliation | Washington University in St. Louis |
| contact email | r.m.searfoss@wustl.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095995/ |
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